ArticleScientific reports2019
Single-Cell Quantification of mRNA Expression in The Human Brain.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- Article
- Isolation of Brain Microglia to Study Persistence of Human Immunodeficiency Virus-1.Journal of visualized experiments : JoVE · 2026Article
- Regional specialization of upper-layer inhibitory interneuron organization in the human prefrontal cortex.Journal of anatomy · 2026Article
- Regional mapping of CSF1R-positive microglia in neurodegenerative diseases and progressive MS, with exploratory presynaptic marker analyses.Journal of neuroinflammation · 2026Article
- Glucagon-Like Peptide-1 Targets in the Human Nodose Ganglion.The Journal of comparative neurology · 2026Article
- Sequence Diversity and Encoded Enzymatic Differences of Monocistronic L1 ORF2 mRNA Variants in the Aged Normal and Alzheimer's Disease Brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Ventral hippocampal parvalbumin interneurons gate the acute anxiolytic action of the serotonergic psychedelic DOI.Neuron · 2024Article
- Studying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2024Article
- Molecular profiling of the hippocampus of children with autism spectrum disorder.Molecular psychiatry · 2024Article
- Temporal and spatial expression analysis of periostin in mice periodontitis model.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2024Article
- Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation.Cell reports · 2024Article
- Article
- Direct Detection of Borrelia Species in Tissues.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Deep learning-based image analysis identifies a DAT-negative subpopulation of dopaminergic neurons in the lateral Substantia nigra.Communications biology · 2023Article
- Neural mechanism of acute stress regulation by trace aminergic signalling in the lateral habenula in male mice.Nature communications · 2023Article
- The development of compulsive coping behaviour is associated with a downregulation of Arc in a Locus Coeruleus neuronal ensemble.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- TREM2 splice isoforms generate soluble TREM2 species that disrupt long-term potentiation.Genome medicine · 2023Article
- Spatial mapping of ectonucleotidase gene expression in the murine urinary bladder.Frontiers in physiology · 2023Article
- Microglial Expression of the Wnt Signaling ModulatoreNeuro · 2023Article
- NeRD: a multichannel neural network to predict cellular response of drugs by integrating multidimensional data.BMC medicine · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
RNA analysis at the cellular resolution in the human brain is challenging. Here, we describe an optimised approach for detecting single RNA transcripts in a cell-type specific manner in frozen human brain tissue using multiplexed fluorescent RNAscope probes. We developed a new robust analytical approach for RNAscope quantification. Our method shows that low RNA integrity does not significantly affect RNAscope signal, recapitulates bulk RNA analysis and provides spatial context to transcriptomic analysis of human post-mortem brain at single-cell resolution. In summary, our optimised method allows the usage of frozen human samples from brain banks to perform quantitative RNAscope analysis.
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