ArticleThe Journal of cell biology2019
Dynamic instability of clathrin assembly provides proofreading control for endocytosis.
Article in The Journal of cell biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Epsin1 enforces a condensation-dependent checkpoint for ubiquitylated cargo during clathrin-mediated endocytosis.Nature communications · 2025Article
- α-Synuclein interacts directly with AP2 and regulates its binding to synaptic membranes.The Journal of biological chemistry · 2025Article
- Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.Cell reports · 2023Article
- Article
- Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.Nature communications · 2022Article
- Article
- Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.Frontiers in molecular biosciences · 2022Article
- De novo endocytic clathrin coats develop curvature at early stages of their formation.Developmental cell · 2021Article
- Nanodissected elastically loaded clathrin lattices relax to increased curvature.Science advances · 2021Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Liquid-like protein interactions catalyse assembly of endocytic vesicles.Nature cell biology · 2021Article
- Functional characterization of 67 endocytic accessory proteins using multiparametric quantitative analysis of CCP dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Spatial regulation of clathrin-mediated endocytosis through position-dependent site maturation.The Journal of cell biology · 2020Article
- Evolving models for assembling and shaping clathrin-coated pits.The Journal of cell biology · 2020Review
- Early and nonredundant functions of dynamin isoforms in clathrin-mediated endocytosis.Molecular biology of the cell · 2020Article
- Reliable estimation of membrane curvature for cryo-electron tomography.PLoS computational biology · 2020Article
- Biophysical forces in membrane bending and traffic.Current opinion in cell biology · 2020Review
- Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.Science advances · 2020Article
- The structures of natively assembled clathrin-coated vesicles.Science advances · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Clathrin-mediated endocytosis depends on the formation of functional clathrin-coated pits that recruit cargos and mediate the uptake of those cargos into the cell. However, it remains unclear whether the cargos in the growing clathrin-coated pits are actively monitored by the coat assembly machinery. Using a cell-free reconstitution system, we report that clathrin coat formation and cargo sorting can be uncoupled, indicating that a checkpoint is required for functional cargo incorporation. We demonstrate that the ATPase Hsc70 and a dynamic exchange of clathrin during assembly are required for this checkpoint. In the absence of Hsc70 function, clathrin assembles into pits but fails to enrich cargo. Using single-molecule imaging, we further show that uncoating takes place throughout the lifetime of the growing clathrin-coated pits. Our results suggest that the dynamic exchange of clathrin, at the cost of the reduced overall assembly rates, primarily serves as a proofreading mechanism for quality control of endocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.