ArticlePloS one2019
Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon).
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Establishment of a cell line from the hematophagous Bat Desmodus rotundus susceptible to Lyssavirus rabies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- The effects of immortalization on the N-glycome and proteome of CDK4-transformed lung cancer cells.Glycobiology · 2024Article
- Establishment of immortalized Egyptian Rousettus bat cell lines.FEBS open bio · 2024Article
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- HPV E6 inhibits E6AP to regulate epithelial homeostasis by modulating keratinocyte differentiation commitment and YAP1 activation.PLoS pathogens · 2023Article
- Immortalization of primary cells derived from the endangered Ryukyu long-furred rat.In vitro cellular & developmental biology. Animal · 2023Article
- Immortalized Canine Adipose-Derived Mesenchymal Stem Cells as a Novel Candidate Cell Source for Mesenchymal Stem Cell Therapy.International journal of molecular sciences · 2023Article
- Immortalization of cells derived from domestic dogs through expressing mutant cyclin-dependent kinase 4, cyclin D1, and telomerase reverse transcriptase.Cytotechnology · 2022Article
- KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media.The journal of poultry science · 2021Article
- Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas.Scientific reports · 2021Article
- Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters.Cytotechnology · 2021Article
- Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells.iScience · 2021Article
- Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor.Frontiers in cell and developmental biology · 2020Article
- Correction: Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon).PloS one · 2020Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.
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