Evidence map›Paper›PMID 31446531›Full record

ArticleMolecular biology reports2019

Selection of reliable reference genes for gene expression analysis in seeds at different developmental stages and across various tissues in Paeonia ostii.

Chaoqiong Li, Lizong Hu, Xueqin Wang, Hongzhan Liu, Huihui Tian, Junsheng Wang

Abstract read
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In one paragraph

Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Chaoqiong Li *College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China. lcq2013@aliyun.com.ORCID http://orcid.org/0000-0001-7152-0263
Lizong Hu *College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Xueqin WangCollege of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Hongzhan LiuCollege of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Huihui TianCollege of Journalism and Media, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Junsheng WangCollege of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China.
Zhoukou Normal University · CN

Funding

National Natural Science Foundation of China 31500534the Key Scientific Research Project in Colleges and Universities of Henan Province 15A180024the Natural Science Foundation of Henan province 162300410346the School-Based Program of Zhoukou Normal University ZKUNB115203the Startup Fund for Advanced Talents of Zhoukou Normal University ZKNU2014108
6 · The paper itself

Abstract

Paeonia ostii seeds have recently been identified as a new source of α-linolenic acid in China. Studying the gene expression patterns of unsaturated fatty acid-related genes would be helpful for understanding the mechanism of α-linolenic acid accumulation. Quantitative real-time polymerase chain reaction (qRT-PCR) is a useful method for reliably evaluating gene expression, and it is necessary to select reliable reference genes for data normalization in qRT-PCR analysis. In this study, we evaluated the expression stability of 12 candidate reference genes using four mathematical algorithms (∆C

Indexed as

Reference StandardsGene ExpressionGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantPaeoniaReal-Time Polymerase Chain ReactionNormalizationPaeonia ostiiqRT-PCRReference geneSeed development

Identifiers

PMID31446531
OpenAlexW2969293482

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.