ArticleThe Journal of cell biology1988
Ifm(2)2 is a myosin heavy chain allele that disrupts myofibrillar assembly only in the indirect flight muscle of Drosophila melanogaster.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- The Mechanisms of Thin Filament Assembly and Length Regulation in Muscles.International journal of molecular sciences · 2022Review
- An ongoing role for structural sarcomeric components in maintaining Drosophila melanogaster muscle function and structure.PloS one · 2014Article
- Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability.Journal of molecular biology · 2010Article
- Loss of Smyhc1 or Hsp90alpha1 function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos.PloS one · 2010Article
- The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis.Development (Cambridge, England) · 2008Article
- Transgenic tools for Drosophila muscle research.Journal of muscle research and cell motility · 2008Review
- Stretchin-klp, a novel Drosophila indirect flight muscle protein, has both myosin dependent and independent isoforms.Journal of muscle research and cell motility · 2005Article
- Assembly of the giant protein projectin during myofibrillogenesis in Drosophila indirect flight muscles.BMC cell biology · 2004Article
- Drosophila muscle regulation characterized by electron microscopy and three-dimensional reconstruction of thin filament mutants.Biophysical journal · 2004Article
- Flightin is essential for thick filament assembly and sarcomere stability in Drosophila flight muscles.The Journal of cell biology · 2000Article
- Interaction of troponin-H and glutathione S-transferase-2 in the indirect flight muscles of Drosophila melanogaster.Journal of muscle research and cell motility · 1998Article
- Growth and muscle defects in mice lacking adult myosin heavy chain genes.The Journal of cell biology · 1997Article
- Ablation of the murine alpha myosin heavy chain gene leads to dosage effects and functional deficits in the heart.The Journal of clinical investigation · 1996Article
- Alterations in flightin phosphorylation in Drosophila flight muscles are associated with myofibrillar defects engendered by actin and myosin heavy-chain mutant alleles.Biochemical genetics · 1994Article
- Transformation of Drosophila melanogaster with the wild-type myosin heavy-chain gene: rescue of mutant phenotypes and analysis of defects caused by overexpression.The Journal of cell biology · 1994Article
- Article
- Flightin, a novel myofibrillar protein of Drosophila stretch-activated muscles.The Journal of cell biology · 1993Article
- Myosin light chain-2 mutation affects flight, wing beat frequency, and indirect flight muscle contraction kinetics in Drosophila.The Journal of cell biology · 1992Article
- Muscle abnormalities in Drosophila melanogaster heldup mutants are caused by missing or aberrant troponin-I isoforms.The Journal of cell biology · 1991Article
- Muscle-specific accumulation of Drosophila myosin heavy chains: a splicing mutation in an alternative exon results in an isoform substitution.The EMBO journal · 1991Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Using a combination of molecular and genetic techniques we demonstrate that Ifm(2)2 is an allele of the single-copy sarcomeric myosin heavy chain gene. Flies homozygous for this allele accumulate wild-type levels of mRNA and protein in tubular muscle of adults, but fail to accumulate detectable amounts of myosin heavy chain mRNA or protein in the indirect flight muscle. We propose that the mutation interferes with either transcription of the gene or splicing of the primary transcript in the indirect flight muscle and not in other muscle tissues. Biochemical and electron microscopic analysis of flies homozygous for this mutation has revealed that thick filament assembly is abolished in the indirect flight muscle resulting in the instability of wild-type thick filament proteins. In contrast, thin filament and Z disc assembly are marginally affected. We discuss a working hypothesis for sarcomere assembly and define and experimental approach to test the predictions of this proposed pathway for sarcomere assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.