Evidence map›Paper›PMID 31444738›Full record

ArticlePurinergic signalling2019

High-resolution detection of ATP release from single cultured mouse dorsal horn spinal cord glial cells and its modulation by noradrenaline.

Varen Eersapah, Sylain Hugel, Rémy Schlichter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Purinergic signalling, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Varen EersapahCentre National de la Recherche Scientifique (UPR3212), Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, 67000, Strasbourg, France.
Sylain HugelCentre National de la Recherche Scientifique (UPR3212), Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, 67000, Strasbourg, France.
Rémy SchlichterCentre National de la Recherche Scientifique (UPR3212), Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, 67000, Strasbourg, France. schlichter@inci-cnrs.unistra.fr.
Institut des Neurosciences Cellulaires et Intégratives · FRUniversité de Strasbourg · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human embryonic kidney 293 (HEK293) cells stably transfected with the rat P2X2 receptor subunit were preincubated with 200 nM progesterone (HEK293-P2X2-PROG), a potent positive allosteric modulator of homomeric P2X2 receptors, and used to detect low nanomolar concentrations of extracellular ATP. Fura-2-loaded HEK293-P2X2-PROG cells were acutely plated on top of cultured DH glial cells to quantify ATP release from single DH glial cells. Application of the α1 adrenoceptor agonist phenylephrine (PHE, 20 μM) or of a low K

Indexed as

Adenosine TriphosphateAnimalsBiosensing TechniquesHEK293 CellsHumansMiceMice, Inbred C57BLNeurogliaNorepinephrineRatsSpinal Cord Dorsal HornAdenosine TriphosphateNorepinephrineAstrocyteExocytosisNon-vesicular releaseSecretory lysosomeVesicular release

Identifiers

PMID31444738
PMCPMC6737151
OpenAlexW2969883150

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.