ArticleNeuroscience bulletin2020
Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing.
Article in Neuroscience bulletin, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- One-step approach producing barcoded rabies virus with optimized diversity.Cell reports methods · 2025Article
- Elucidation of the binding interaction interface between AAV serotype 11 capsid protein and host nuclear import proteins.Molecular therapy. Methods & clinical development · 2025Article
- Article
- Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model.Animal models and experimental medicine · 2025Article
- Direct Hippocampal and Thalamic Inputs to Layer 3 Pyramidal Cells in the Medial Entorhinal Cortex Revealed by Monosynaptic Rabies Tracing.Neuroscience bulletin · 2025Article
- Vulnerability of long-range inputome of basal forebrain in normal aging mice.Frontiers in aging neuroscience · 2025Article
- Article
- Virus-Based Neural Circuit Tracing.Advances in neurobiology · 2024Review
- In Vivo Neural Tract Tracing Procedures: Unravelling Neural Hodology Using Fluorescent and Non-fluorescent Neural Tract Tracers, Optogenetic Approach, and Diffusion Tensor Neurotractography Protocols.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- The locus coeruleus directs sensory-motor reflex amplitude across environmental contexts.Current biology : CB · 2023Article
- Genomic stability of self-inactivating rabies.eLife · 2023Article
- A Novel Mouse Model for Polysynaptic Retrograde Tracing and Rabies Pathological Research.Cellular and molecular neurobiology · 2023Article
- A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing.Neural regeneration research · 2023Article
- AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction.Nature communications · 2023Article
- Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imagingiScience · 2023Article
- Organizational Principles of the Centrifugal Projections to the Olfactory Bulb.International journal of molecular sciences · 2023Article
- Viral Tools for Neural Circuit Tracing.Neuroscience bulletin · 2022Review
- A new anterograde trans-synaptic tracer based on Sindbis virus.Neural regeneration research · 2022Article
- Lighting Up Neural Circuits by Viral Tracing.Neuroscience bulletin · 2022Review
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2C glycoprotein (SAD-RV(ΔG)-N2C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized. We compared these features in different mouse brain regions for SAD-RV-N2C(G) and two other widely-used retrograde tracers, SAD-RV(ΔG)-B19(G) and rAAV2-retro. We found that SAD-RV(ΔG)-N2C(G) enhanced the infection efficiency of long-projecting neurons by ~10 times but with very similar neuro-tropism, compared with SAD-RV(ΔG)-B19(G). On the other hand, SAD-RV(ΔG)-N2C(G) had an infection efficiency comparable with rAAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of long-projecting neuronal populations. These results demonstrate that SAD-RV(ΔG)-N2C(G) can serve as an effective retrograde vector for studying neuronal circuits.
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