ArticleScientific reports2019
Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.Journal of orthopaedic translation · 2026Review
- A modular platform for automated organoid culture and longitudinal imaging.Scientific reports · 2026Article
- Advancements in theFrontiers in toxicology · 2025Review
- The technology, opportunities, and challenges of Synthetic Biological Intelligence.Biotechnology advances · 2023Review
- Suspension culture on microcarriers and as aggregates enables expansion and differentiation of pluripotent stem cells (PSCs).Cytotherapy · 2023Article
- Preclinical Research of Stem Cells: Challenges and Progress.Stem cell reviews and reports · 2023Review
- Automating iPSC generation to enable autologous photoreceptor cell replacement therapy.Journal of translational medicine · 2023Article
- Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration.Bioactive materials · 2023Review
- Dynamic Culture of Mesenchymal Stromal/Stem Cell Spheroids and Secretion of Paracrine Factors.Frontiers in bioengineering and biotechnology · 2022Review
- Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.Stem cell reports · 2021Article
- High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling.Stem cells translational medicine · 2021Article
- Advances in removing mass transport limitations for more physiologically relevantBiophysics reviews · 2021Review
- Engineered Microsystems for Spheroid and Organoid Studies.Advanced healthcare materials · 2021Review
- Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells.bioRxiv : the preprint server for biology · 2020Article
- End-to-End Platform for Human Pluripotent Stem Cell Manufacturing.International journal of molecular sciences · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring options and emerging automated process control strategies allow for the necessary highly defined culture conditions. Next to standard process characteristics such as oxygen consumption, pH, and metabolite turnover, a reproducible and steady formation of hiPSC aggregates is vital for process scalability. In this regard, we developed a hiPSC-specific suspension culture unit consisting of a fully monitored CSTR system integrated into a custom-designed and fully automated incubator. As a step towards cost-effective hiPSC suspension culture and to pave the way for flexibility at a large scale, we constructed and utilized tailored miniature CSTRs that are largely made from three-dimensional (3D) printed polylactic acid (PLA) filament, which is a low-cost material used in fused deposition modelling. Further, the monitoring tool for hiPSC suspension cultures utilizes in situ microscopic imaging to visualize hiPSC aggregation in real-time to a statistically significant degree while omitting the need for time-intensive sampling. Suitability of our culture unit, especially concerning the developed hiPSC-specific CSTR system, was proven by demonstrating pluripotency of CSTR-cultured hiPSCs at RNA (including PluriTest) and protein level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.