ArticleScientific reports2019
Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 79 citations in OpenAlex.
- Cell-Mediated Mechanical Stability Enhancement of Biomimetic Collagen-Alginate Hydrogels: A Mechanistic Study on the Two-Dimensional Extracellular Matrix-Cell Interaction.Chemistry of materials : a publication of the American Chemical Society · 2026Article
- Understanding sub-cellular mechanochemical correlations of the neonatal extrahepatic bile duct extracellular matrix by atomic force microscope-Infrared spectroscopy.Analytica chimica acta · 2026Article
- Indenting multicellular spheroids with various tip geometries.European biophysics journal : EBJ · 2026Article
- FCHo2, not talin, enables inside-out activation of integrin ɑvβ5 in curved adhesions.Nature communications · 2026Article
- Development of a 3D In Vitro Model of Dupuytren's Disease as a Platform for Drug Screening.Cellular and molecular bioengineering · 2026Article
- Mesenchymal stem cell-derived extracellular matrix for musculoskeletal tissue regeneration.Communications biology · 2026Review
- Review
- FCHo2, instead of talin, enables inside-out activation of integrin ɑvβ5 in curved adhesions.bioRxiv : the preprint server for biology · 2025Article
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
- Optimizations of Placenta Extracellular Matrix-Loaded Silk Fibroin/Alginate 3D-Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering.Engineering in life sciences · 2025Article
- Morphological and Biophysical Study of S100A9 Protein Fibrils by Atomic Force Microscopy Imaging and Nanomechanical Analysis.Biomolecules · 2024Article
- Exploiting Matrix Stiffness to Overcome Drug Resistance.ACS biomaterials science & engineering · 2024Review
- Tumor biomechanical stiffness by magnetic resonance elastography predicts surgical outcomes and identifies biomarkers in vestibular schwannoma and meningioma.Scientific reports · 2024Article
- Unexpected softening of a fibrous matrix by contracting inclusions.Acta biomaterialia · 2024Article
- Atomic force microscopy for characterization of decellularized extracellular matrix (dECM) based materials.Science and technology of advanced materials · 2024Review
- Preparation and Microscopic Mechanical Characterization of L-Methionine-Based Polyphosphazene Fibrous Mats for Vascular Tissue Engineering.Pharmaceutics · 2023Article
- Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions.Nature cell biology · 2023Article
- The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023Review
- Correlation between biological and mechanical properties of extracellular matrix from colorectal peritoneal metastases in human tissues.Scientific reports · 2023Article
- The Extracellular Matrix: Its Composition, Function, Remodeling, and Role in Tumorigenesis.Biomimetics (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular matrix (ECM), as a dynamic component of the tissue, influences cell behavior and plays an important role in cell mechanics and tissue homeostasis. Reciprocally, this three-dimensional scaffold is dynamically, structurally and mechanically modified by cells. In the field of biophysics, the independent role of cell and ECM mechanics has been largely investigated; however, there is a lack of experimental data reporting the interdependent interplay between cell and ECM mechanics, measured simultaneously. Here, using Atomic Force Microscopy (AFM) we have characterized five different decellularized matrices diverse in their topography, ECM composition and stiffness and cultured them with normal and pathological fibroblasts (scar and Dupuytren's). We investigated the change in topography and elasticity of these matrices due to cell seeding, by using AFM peak force imaging and mechanical mapping, respectively. We found normal fibroblasts soften these matrices more than pathological fibroblasts, suggesting that pathological fibroblasts are profoundly influencing tissue stiffening in fibrosis. We detected different ECM composition of decellularized matrices used here influences fibroblast stiffness, thus highlighting that cell mechanics not only depends on ECM stiffness but also on their composition. We used confocal microscopy to assess fibroblasts invasion and found pathological fibroblasts were invading the matrices deeper than normal fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.