ArticleNature communications2019
Self-organized formation of developing appendages from murine pluripotent stem cells.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Article
- Generation of Budoids: 3D Multilineage Limb Models From Mouse Embryonic Stem Cells.Bio-protocol · 2026Article
- Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model.Science advances · 2025Article
- Modeling of skeletal development and diseases using human pluripotent stem cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Review
- Classes of Stem Cells: From Biology to Engineering.Regenerative engineering and translational medicine · 2024Article
- Direct reprogramming of non-limb fibroblasts to cells with properties of limb progenitors.Developmental cell · 2024Article
- Human-Induced Pluripotent Stem Cells in Plastic and Reconstructive Surgery.International journal of molecular sciences · 2024Review
- Lgr6-expressing functional nail stem-like cells differentiated from human-induced pluripotent stem cells.PloS one · 2024Article
- Limb blastema formation: How much do we know at a genetic and epigenetic level?The Journal of biological chemistry · 2023Review
- How might we build limbsFrontiers in cell and developmental biology · 2023Review
- Identification of Surface Antigens That Define Human Pluripotent Stem Cell-Derived PRRX1+Limb-Bud-like Mesenchymal Cells.International journal of molecular sciences · 2022Article
- Recombinant Limb Assay asFrontiers in cell and developmental biology · 2022Article
- M2c Macrophages enhance phalange regeneration of amputated mice digits in an organ co-culture system.Iranian journal of basic medical sciences · 2021Article
- Induction and expansion of human PRRX1Nature biomedical engineering · 2021Article
- Appendage regeneration is context dependent at the cellular level.Open biology · 2021Article
- Review
- The Progress of Stem Cell Technology for Skeletal Regeneration.International journal of molecular sciences · 2021Review
- Tissue Engineering for Musculoskeletal Regeneration and Disease Modeling.Handbook of experimental pharmacology · 2021Article
- Understanding paraxial mesoderm development and sclerotome specification for skeletal repair.Experimental & molecular medicine · 2020Review
- Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Limb development starts with the formation of limb buds (LBs), which consist of tissues from two different germ layers; the lateral plate mesoderm-derived mesenchyme and ectoderm-derived surface epithelium. Here, we report means for induction of an LB-like mesenchymal/epithelial complex tissues from murine pluripotent stem cells (PSCs) in vitro. The LB-like tissues selectively differentiate into forelimb- or hindlimb-type mesenchymes, depending on a concentration of retinoic acid. Comparative transcriptome analysis reveals that the LB-like tissues show similar gene expression pattern to that seen in LBs. We also show that manipulating BMP signaling enables us to induce a thickened epithelial structure similar to the apical ectodermal ridge. Finally, we demonstrate that the induced tissues can contribute to endogenous digit tissue after transplantation. This PSC technology offers a first step for creating an artificial limb bud in culture and might open the door to inducing other mesenchymal/epithelial complex tissues from PSCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.