ArticleNature communications2019
Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 138 citations in OpenAlex.
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- Toward sustainable production of bulnesol and elemol enabled by synthetic biology in Saccharomyces cerevisiae.Archives of microbiology · 2026Article
- The comprehensive review of eucalyptol: synthesis, metabolism, and therapeutic applications in disease treatment.Molecular biology reports · 2025Review
- Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons.Nature communications · 2025Article
- The cis-prenyltransferase protein family in Taraxacum koksaghyz.The Plant journal : for cell and molecular biology · 2025Article
- Engineering cellular dephosphorylation boosts (+)-borneol production in yeast.Acta pharmaceutica Sinica. B · 2025Article
- Engineering Saccharomyces cerevisiae for medical applications.Microbial cell factories · 2025Review
- Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock.Biotechnology for biofuels and bioproducts · 2024Article
- Introducing carbon assimilation in yeasts using photosynthetic directed endosymbiosis.Nature communications · 2024Article
- Efficient synthesis of limonene production in Yarrowia lipolytica by combinatorial engineering strategies.Biotechnology for biofuels and bioproducts · 2024Article
- Engineering a carbon source-responsive promoter for improved biosynthesis in the non-conventional yeastMetabolic engineering communications · 2024Article
- Engineering peroxisomal biosynthetic pathways for maximization of triterpene production inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- De novo biosynthesis of the hops bioactive flavonoid xanthohumol in yeast.Nature communications · 2024Article
- Brewing coral terpenes-A yeast based approach to soft coral terpene cyclases.Methods in enzymology · 2024Article
- Widespread biosynthesis of 16-carbon terpenoids in bacteria.Nature chemical biology · 2023Article
- Biomimetic Approach to Diverse Coral Diterpenes from a Biosynthetic Scaffold.Angewandte Chemie (International ed. in English) · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology efforts for the production of valuable chemicals are frequently hindered by the structure and regulation of the native metabolic pathways of the chassis. This is particularly evident in the case of monoterpenoid production in Saccharomyces cerevisiae, where the canonical terpene precursor geranyl diphosphate is tightly coupled to the biosynthesis of isoprenoid compounds essential for yeast viability. Here, we establish a synthetic orthogonal monoterpenoid pathway based on an alternative precursor, neryl diphosphate. We identify structural determinants of isomeric substrate selectivity in monoterpene synthases and engineer five different enzymes to accept the alternative substrate with improved efficiency and specificity. We combine the engineered enzymes with dynamic regulation of metabolic flux to harness the potential of the orthogonal substrate and improve the production of industrially-relevant monoterpenes by several-fold compared to the canonical pathway. This approach highlights the introduction of synthetic metabolism as an effective strategy for high-value compound production.
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