ReviewProteomes2019
What is Normalization? The Strategies Employed in Top-Down and Bottom-Up Proteome Analysis Workflows.
Review in Proteomes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Protein-level batch-effect correction enhances robustness in MS-based proteomics.Nature communications · 2025Article
- Cannabinoid Receptor Type 2 Agonist JWH-133 Stimulates Antiviral Factors and Decreases Proviral, Inflammatory, and Neurotoxic Proteins in HIV-Infected Macrophage Secretome.International journal of molecular sciences · 2025Article
- Epitranscriptomic analysis reveals clinical and molecular signatures in glioblastoma.Acta neuropathologica communications · 2025Article
- Maximizing Glycoproteomics Results through an Integrated Parallel Accumulation Serial Fragmentation Workflow.Analytical chemistry · 2024Article
- Proteomics-The State of the Field: The Definition and Analysis of Proteomes Should Be Based in Reality, Not Convenience.Proteomes · 2024Article
- Total serum pentosidine quantification using liquid chromatography-tandem mass spectrometry.Bone reports · 2024Article
- Cytokine Profiling of Plasma and Atherosclerotic Plaques in Patients Undergoing Carotid Endarterectomy.International journal of molecular sciences · 2024Article
- Integrative multi-omics and systems bioinformatics in translational neuroscience: A data mining perspective.Journal of pharmaceutical analysis · 2023Review
- Targeted Quantification of Protein Phosphorylation and Its Contributions towards Mathematical Modeling of Signaling Pathways.Molecules (Basel, Switzerland) · 2023Review
- A Bioconductor workflow for processing, evaluating, and interpreting expression proteomics data.F1000Research · 2023Article
- Multivariate Analysis with the R Package mixOmics.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A community resource to mass explore the wheat grain proteome and its application to the late-maturity alpha-amylase (LMA) problem.GigaScience · 2022Article
- Article
- Cancer glycomics offers potential biomarkers and therapeutic targets in the framework of 3P medicine.Frontiers in endocrinology · 2022Review
- Distinct systemic and mucosal immune responses during acute SARS-CoV-2 infection.Nature immunology · 2021Article
- Subcellular proteomics.Nature reviews. Methods primers · 2021Article
- Special Issue "Top-down Proteomics: In Memory of Dr Alfred Yergey". Alfred Linwood Yergey, III, 17 September 1941-27 May 2018.Proteomes · 2020Article
- An Integrated Quantitative Proteomics Workflow for Cancer Biomarker Discovery and Validation in Plasma.Frontiers in oncology · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accurate quantification of changes in the abundance of proteins is one of the main applications of proteomics. The maintenance of accuracy can be affected by bias and error that can occur at many points in the experimental process, and normalization strategies are crucial to attempt to overcome this bias and return the sample to its regular biological condition, or normal state. Much work has been published on performing normalization on data post-acquisition with many algorithms and statistical processes available. However, there are many other sources of bias that can occur during experimental design and sample handling that are currently unaddressed. This article aims to cast light on the potential sources of bias and where normalization could be applied to return the sample to its normal state. Throughout we suggest solutions where possible but, in some cases, solutions are not available. Thus, we see this article as a starting point for discussion of the definition of and the issues surrounding the concept of normalization as it applies to the proteomic analysis of biological samples. Specifically, we discuss a wide range of different normalization techniques that can occur at each stage of the sample preparation and analysis process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.