Evidence map›Paper›PMID 31440304›Full record

ArticleNature photonics2019

High-resolution, high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy.

Junhui Shi, Terence T W Wong, Yun He, Lei Li, Ruiying Zhang, Christopher S Yung, Jeeseong Hwang, Konstantin Maslov, Lihong V Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature photonics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.

0numbers the graph read from it
0cells of the map it votes in
94citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

94 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Label-Free 3D Photoacoustic Imaging of Tumor Organoids for Volumetric Drug Screening.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

34 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Junhui ShiCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Terence T W WongCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Yun HeCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Lei LiCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Ruiying ZhangOptical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Christopher S YungApplied Physics Division, National Institute of Standards and Technology, 325 Broadway Street, Boulder, CO 80305, USA.
Jeeseong HwangApplied Physics Division, National Institute of Standards and Technology, 325 Broadway Street, Boulder, CO 80305, USA.
Konstantin MaslovCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Lihong V WangCaltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Funding

PHOTON TUNNELING: SHEDDING NEW LIGHT ON BIOMEDICINEDP1EB016986 · NIBIB · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2012 to 2016
$3.8M
IN VIVO IMAGING OF SINGLE CIRCULATING CELLSR01CA186567 · NCI · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2013 to 2017
$3.6M
Deep brain photoacoustic tomography at single-neuron resolution using arrays of photonic emitters and high-frequency ultrasound transducersU01NS099717 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI ROUKES, MICHAEL L, SHEPARD, KENNETH L · 2016 to 2018
$3.0M
FAST HIGH-RESOLUTION DEEP PHOTOACOUSTIC TOMOGRAPHY OF ACTION POTENTIALS IN BRAINSU01NS090579 · NINDS · WASHINGTON UNIVERSITY · PI WANG, LIHONG · 2014 to 2016
$2.7M
NCI NIH HHS R01 CA186567NIBIB NIH HHS DP1 EB016986NINDS NIH HHS U01 NS090579NINDS NIH HHS U01 NS099717
6 · The paper itself

Abstract

Mid-infrared (MIR) microscopy provides rich chemical and structural information about biological samples, without staining. Conventionally, the long MIR wavelength severely limits the lateral resolution owing to optical diffraction; moreover, the strong MIR absorption of water ubiquitous in fresh biological samples results in high background and low contrast. To overcome these limitations, we propose a method that employs photoacoustic detection highly localized with a pulsed ultraviolet (UV) laser on the basis of the Grüneisen relaxation effect. For cultured cells, our method achieves water-background suppressed MIR imaging of lipids and proteins at UV resolution, at least an order of magnitude finer than the MIR diffraction limits. Label-free histology using this method is also demonstrated in thick brain slices. Our approach provides convenient high-resolution and high-contrast MIR imaging, which can benefit diagnosis of fresh biological samples.

Identifiers

PMID31440304
PMCPMC6705424

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.