ArticleFrontiers in plant science2019
Single Primer Enrichment Technology (SPET) for High-Throughput Genotyping in Tomato and Eggplant Germplasm.
Article in Frontiers in plant science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed.
- The FORGENIUS Genomic Resources: New Genotyping Tools and Genomic Data for 23 Forest Tree Species and Their Genetic Conservation Units.Molecular ecology resources · 2026Article
- Non-Authentic Genotypes-An Unrevealed Problem in Plant Research and Breeding.Plants (Basel, Switzerland) · 2026Article
- A neofunctionalized flowering antagonist created an evolutionary contingency that channeled Solanaceae adaptation.bioRxiv : the preprint server for biology · 2026Article
- A tomato MAGIC population reveals candidate genes for leaf dry matter and phenolics, two key traits for stress resilience and climate-smart breeding.Frontiers in plant science · 2026Article
- Harnessing the untapped genetic diversity of local landraces: omics technologies as a gateway to horticultural breeding.Frontiers in plant science · 2026Review
- A comprehensive SPET-based linkage mapping for berry texture and defective seed development in grapevine.Frontiers in plant science · 2026Article
- Article
- Effects of genotype × environment interactions on the morphological and genetic aspects of the seed characteristics of faba beans.The plant genome · 2025Article
- Graph-based pangenomes and pan-phenome provide a cornerstone for eggplant biology and breeding.Nature communications · 2025Article
- Genome-wide association study of agronomic traits in winter wheat (Triticum aestivum L.) using a custom SNP marker set.BMC plant biology · 2025Article
- Resolving a century-old enigma: potato 'Bolters' originate from instability of the StCDF1.3 allele.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Resequencing and phenotyping of the first highly inbred eggplant multiparent population revealHorticulture research · 2025Article
- Benchmarking of low coverage sequencing workflows for precision genotyping in eggplant.BMC plant biology · 2025Article
- Association analyses reveal both anthropic and environmental selective events during eggplant domestication.The Plant journal : for cell and molecular biology · 2025Article
- Tissue-specific resistance and susceptibility to the tomato brown rugose fruit virus (ToBRFV) conferred by Solanum pennellii loci.BMC plant biology · 2025Article
- Conservation and valorization of Italian potato biodiversity through genotype-by-sequencing (GBS).Frontiers in plant science · 2025Article
- Irregular green netting of eggplant fruit peel: a domestication trait controlled by SmGLK2 with potential for fruit colour diversification.Journal of experimental botany · 2024Article
- The Jan Sjödin faba bean mutant collection: morphological and molecular characterization.Hereditas · 2024Article
- Convergent evolution of plant prickles by repeated gene co-option over deep time.Science (New York, N.Y.) · 2024Article
- A novel tomato interspecific (Horticulture research · 2024Article
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Authors and funding
18 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Single primer enrichment technology (SPET) is a new, robust, and customizable solution for targeted genotyping. Unlike genotyping by sequencing (GBS), and like DNA chips, SPET is a targeted genotyping technology, relying on the sequencing of a region flanking a primer. Its reliance on single primers, rather than on primer pairs, greatly simplifies panel design, and allows higher levels of multiplexing than PCR-based genotyping. Thanks to the sequencing of the regions surrounding the target SNP, SPET allows the discovery of thousands of closely linked, novel SNPs. In order to assess the potential of SPET for high-throughput genotyping in plants, a panel comprising 5k target SNPs, designed both on coding regions and introns/UTRs, was developed for tomato and eggplant. Genotyping of two panels composed of 400 tomato and 422 eggplant accessions, comprising both domesticated material and wild relatives, generated a total of 12,002 and 30,731 high confidence SNPs, respectively, which comprised both target and novel SNPs in an approximate ratio of 1:1.6, and 1:5.5 in tomato and eggplant, respectively. The vast majority of the markers was transferrable to related species that diverged up to 3.4 million years ago (
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