ArticleScientific reports2019
The Zea mays mutants opaque2 and opaque16 disclose lysine change in waxy maize as revealed by RNA-Seq.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 25 citations in OpenAlex.
- Maize Biofortification Approaches for Fighting Malnutrition in Sub-Saharan Africa.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- Dynamics of accumulation of lysine and tryptophan, and kernel modification in opaque2, opaque16, and double mutant (opaque2/opaque16) genotypes during kernel development in maize.Journal of applied genetics · 2025Article
- Multiomics of a rice population identifies genes and genomic regions that bestow low glycemic index and high protein content.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramidingBreeding science · 2024Article
- Assessing sequence variation, haplotype analysis and molecular characterisation of aspartate kinase2 (ask2) gene regulating methionine biosynthesis in diverse maize inbreds.Molecular genetics and genomics : MGG · 2024Article
- A comprehensive review onFrontiers in genetics · 2024Review
- Metabolomics and transcriptomics strategies to reveal the mechanism of diversity of maize kernel color and quality.BMC genomics · 2023Article
- Comparison of the physicochemical properties of starches from maize reciprocal F1 hybrids and their parental lines.Food chemistry: X · 2023Article
- Genotypic Variation of Nitrogen Use Efficiency and Amino Acid Metabolism in Barley.Frontiers in plant science · 2021Article
- Marker aided introgression ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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7 authors at 2 institutions in 1 country.
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Abstract
In maize, opaque2 (o2) and opaque16 (o16) alleles can increase lysine content, while the waxy (wx) gene can enhance the amylopectin content of grains. In our study, o2 and o16 alleles were backcrossed into waxy maize line (wxwx). The o2o2o16o16wxwx lines had amylopectin contents similar to those of waxy line. Their nutritional value was better than waxy line, but the mechanism by which the o2 and o16 alleles increased the lysine content of waxy maize remained unclear. The o2o2o16o16wxwx lines and their parents on kernels (18th day after pollination) were subjected to RNA sequencing (RNA-Seq). The RNA-Seq analysis revealed 272 differentially expressed genes (DEGs). Functional analyses revealed that these DEGs were mainly related to biomass metabolism. Among them, in o2o2o16o16wxwx lines, 15 genes encoding α-zein were down-regulated, which resulted in the reduction of α-zein synthesis and increased lysine content; lkr/sdh1 and Zm00001d020984.1 genes involved in the lysine degradation pathway were down-regulated, thereby inhibited lysine degradation; sh2, bt2 and ae1 genes involved in starch metabolism were upregulated, leaded to wrinkling kernel and farinaceous endosperm. Our transcriptional-level identification of key genes responsible for increased grain lysine content and farinaceous endosperm formation following introgression of o2 and o16 alleles should promote molecular breeding for maize quality.
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