ArticleRadiation oncology (London, England)2019
Development of a high-throughput γ-H2AX assay based on imaging flow cytometry.
Article in Radiation oncology (London, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 2 syntheses or guidelines pooled it, 106 citations in OpenAlex.
- Research progress on the role and mechanism of DNA damage repair in germ cell development.Frontiers in endocrinology · 2023Pooled it
- DNA repair phenotype and cancer risk: a systematic review and meta-analysis of 55 case-control studies.Scientific reports · 2022Pooled it
- Relationship between radiosensitivity of peripheral blood lymphocytes, breast cancer cells, and breast cancer stem cells in patients with breast cancer.Biochemistry and biophysics reports · 2026Article
- Assessment of cytotoxicity and genomic instability induced by coal-derived nanoparticles in V79 and HaCaT Cells.Archives of toxicology · 2026Article
- Spontaneous aging-associated inflammation and genome instability in the immune system of turquoise killifish.Nature aging · 2026Article
- Automated Intracellular Immunofluorescence Staining Enabled by Magnetic 3D Mixing in a Modular Microfluidic Platform.Biosensors · 2026Article
- Application of an imaging flow cytometry γ-H2AX assay for biodosimetry using supervised machine learning.International journal of radiation biology · 2026Article
- Dynamic γ-H2AX response in blood lymphocytes for prediction of radiotherapy induced bladder toxicity in cervical cancer patients.The Indian journal of medical research · 2026Article
- Microfluidic Isolation of Aptamers for Intracellular Measurement of Radio-Responsive Proteins.Radiation (Multidisciplinary Digital Publishing Institute) · 2025Article
- Comprehensive Measurement of Inter-Individual Variation in DNA Repair Capacity in Healthy Individuals.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Quantitative Analysis of DNA Double-Strand Breaks in Genomic DNA Using Standard Curve Method.Journal of clinical laboratory analysis · 2025Article
- Review
- Centers for Medical Countermeasures against Radiation Consortium: Past, Present, and Beyond.Radiation research · 2025Review
- Robotic radiation shielding system reduces radiation-induced DNA damage in operators performing electrophysiological procedures.Scientific reports · 2025Article
- Advancing cell-free DNA as a biomarker of damage to heart caused by ionizing radiation.Journal of radiation research · 2025Article
- Revisiting the approaches to DNA damage detection in genetic toxicology: insights and regulatory implications.BioData mining · 2025Article
- Predictive value of 8-hydroxy-2'-deoxyguanosine and 8-nitroguanine production in radiation-induced skin damage after postoperative breast cancer radiotherapy.Exploration of targeted anti-tumor therapy · 2025Article
- The effects of carbon-ion beam irradiation on three-dimensional in vitro models of normal oral mucosa and oral cancer: development of a novel tool to evaluate cancer therapy.In vitro cellular & developmental biology. Animal · 2024Article
- Impact of Proton Irradiation Depending on Breast Cancer Subtype in Patient-Derived Cell Lines.International journal of molecular sciences · 2024Article
- Microfluidic measurement of intracellular mRNA with a molecular beacon probe towards point-of-care radiation triage.Sensors & diagnostics · 2024Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundMeasurement of γ-H2AX foci levels in cells provides a sensitive and reliable method for quantitation of the radiation-induced DNA damage response. The objective of the present study was to develop a rapid, high-throughput γ-H2AX assay based on imaging flow cytometry (IFC) using the ImageStream®
methodsThe γ-H2AX protocol was developed and optimized for small volumes (100 μL) of human blood in Matrix™ 96-tube format. Blood cell lymphocytes were identified and captured by ISX INSPIRE™ software and analyzed by Data Exploration and Analysis Software.
resultsDose- and time-dependent γ-H2AX levels corresponding to radiation exposure were measured at various time points over 24 h using the IFC system. γ-H2AX fluorescence intensity at 1 h after exposure, increased linearly with increasing radiation dose (R
conclusionsThe results indicate that the IFC-based γ-H2AX protocol may provide a practical and high-throughput platform for measurements of individual global DNA DSB repair capacity which can facilitate precision medicine by predicting individual radiosensitivity and risk of developing adverse effects related to radiotherapy treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.