ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2019
Regulatory mechanisms underlying the specification of the pupal-homologous stage in a hemimetabolous insect.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects.Communications biology · 2026Article
- Evolution of complete metamorphosis through temporal shifts in Chronologically inappropriate morphogenesis (Chinmo) and Broad.Development (Cambridge, England) · 2026Article
- Chinmo function in cockroaches provides new insights into the regulation and evolution of insect metamorphosis.PLoS genetics · 2025Article
- When and how do 17-year periodical cicada nymphs decide to emerge? A field test of the 4-year-gate hypothesis.Proceedings. Biological sciences · 2025Article
- Article
- E93 controls adult differentiation by repressingProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Developmental correspondence of juvenile stages across the locust, harlequin ladybird, and diamondback moth.iScience · 2024Article
- Article
- Knockdown ofBiology · 2022Article
- Molecular mechanisms underlying metamorphosis in the most-ancestral winged insect.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Drosophila E93 promotes adult development and suppresses larval responses to ecdysone during metamorphosis.Developmental biology · 2022Article
- RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (International journal of molecular sciences · 2020Article
- Precocious Downregulation of Krüppel-Homolog 1 in the Migratory Locust,International journal of molecular sciences · 2020Article
- The evolution of insect metamorphosis: a developmental and endocrine view.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Complete metamorphosis of insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Cooking up the perfect insect: Aristotle's transformational idea about the complete metamorphosis of insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Evaluating responses to temperature during pre-metamorphosis and carry-over effects at post-metamorphosis in the wood tiger moth (Arctia plantaginis).Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Where did the pupa come from? The timing of juvenile hormone signalling supports homology between stages of hemimetabolous and holometabolous insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- The innovation of the final moult and the origin of insect metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Juvenile hormones and the genetic interaction between the transcription factors Krüppel homologue 1 (Kr-h1) and Broad (Br) regulate the transformation of insects from immature to adult forms in both types of metamorphosis (holometaboly with a pupal stage versus hemimetaboly with no pupal stage); however, knowledge about the exact instar in which this occurs is limited. Using the hemimetabolous cricket Gryllus bimaculatus (Gb), we demonstrate that a genetic interaction occurs among Gb'Kr-h1, Gb'Br and the adult-specifier transcription factor Gb'E93 from the sixth to final (eighth) nymphal instar. Gb'Kr-h1 and Gb'Br mRNAs were strongly expressed in the abdominal tissues of sixth instar nymphs, with precocious adult moults being induced by Gb'Kr-h1 or Gb'Br knockdown in the sixth instar. The depletion of Gb'Kr-h1 or Gb'Br upregulates Gb'E93 in the sixth instar. By contrast, Gb'E93 knockdown at the sixth instar prevents nymphs transitioning to adults, instead producing supernumerary nymphs. Gb'E93 also represses Gb'Kr-h1 and Gb'Br expression in the penultimate nymphal instar, demonstrating its important role in adult differentiation. Our results suggest that the regulatory mechanisms underlying the pupal transition in holometabolous insects are evolutionarily conserved in hemimetabolous G. bimaculatus, with the penultimate and final nymphal periods being equivalent to the pupal stage. This article is part of the theme issue 'The evolution of complete metamorphosis'.
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