Evidence map›Paper›PMID 31438568›Full record

ArticleJournal of clinical medicine2019

Systematic Evaluation of Light-Activatable Biohybrids for Anti-Glioma Photodynamic Therapy.

Collin T Inglut, Yan Baglo, Barry J Liang, Yahya Cheema, Jillian Stabile, Graeme F Woodworth, Huang-Chiao Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
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  16. Article
  17. Review
  18. Photodynamic Priming Modulates Endothelial Cell-Cell Junction Phenotype for Light-activated Remote Control of Drug Delivery.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Collin T InglutFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Yan BagloFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Barry J LiangFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Yahya CheemaFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Jillian StabileFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Graeme F WoodworthDepartment of Neurosurgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Huang-Chiao HuangFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA. hchuang@umd.edu.

Funding

Multifunctional, GBM-Activatable Nanocarriers For Image-Guided PhotochemotherapyR00CA194269 · NCI · UNIV OF MARYLAND, COLLEGE PARK · PI HUANG, HUANG CHIAO · 2018 to 2020
$701k
NCI NIH HHS R00 CA194269NCI NIH HHS R00CA194269
6 · The paper itself

Abstract

Photosensitizing biomolecules (PSBM) represent a new generation of light-absorbing compounds with improved optical and physicochemical properties for biomedical applications. Despite numerous advances in lipid-, polymer-, and protein-based PSBMs, their effective use requires a fundamental understanding of how macromolecular structure influences the physicochemical and biological properties of the photosensitizer. Here, we prepared and characterized three well-defined PSBMs based on a clinically used photosensitizer, benzoporphyrin derivative (BPD). The PSBMs include 16:0 lysophosphocholine-BPD (16:0 Lyso PC-BPD), distearoyl-phosphoethanolamine-polyethylene-glycol-BPD (DSPE-PEG-BPD), and anti-EGFR cetuximab-BPD (Cet-BPD). In two glioma cell lines, DSPE-PEG-BPD exhibited the highest singlet oxygen yield but was the least phototoxic due to low cellular uptake. The 16:0 Lyso PC-BPD was most efficient in promoting cellular uptake but redirected BPD's subcellular localization from mitochondria to lysosomes. At 24 h after incubation, proteolyzed Cet-BPD was localized to mitochondria and effectively disrupted the mitochondrial membrane potential upon light activation. Our results revealed the variable trafficking and end effects of PSBMs, providing valuable insights into methods of PSBM evaluation, as well as strategies to select PSBMs based on subcellular targets and cytotoxic mechanisms. We demonstrated that biologically informed combinations of PSBMs to target lysosomes and mitochondria, concurrently, may lead to enhanced therapeutic effects against gliomas.

Indexed as

benzoporphyrin derivativebrain cancerphotodynamic therapyphotosensitizing biomoleculesubcellular targeting

Identifiers

PMID31438568
PMCPMC6780262

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.