ArticleComparative immunology, microbiology and infectious diseases2019
Molecular characterization of Giardia intestinalis and Cryptosporidium parvum from calves with diarrhoea in Austria and evaluation of point-of-care tests.
Article in Comparative immunology, microbiology and infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals.PLoS neglected tropical diseases · 2025Pooled it
- Seasonal Prevalence and Molecular Characterization ofLife (Basel, Switzerland) · 2026Article
- Article
- Importance and Characterisation of Concurrent Pathogens in Diarrhoeic Calves from North-Western Spain.Animals : an open access journal from MDPI · 2025Article
- Coprological and molecular prevalence of Cryptosporidium and Giardia in cattle and irrigation water from Beni-Suef Governorate, Egypt.Scientific reports · 2025Article
- Establishment of an in vitro co-infection model of Cryptosporidium parvum and Giardia duodenalis.Parasites & vectors · 2025Article
- Prevalence ofInternational journal of veterinary science and medicine · 2025Article
- Prevalence and infestation dynamics of intestinal protozoa infections (cryptosporidiosis, giardiasis and eimeriosis) among calves in dairy farms of Kazakhstan.Frontiers in veterinary science · 2025Article
- Article
- Examining the molecular epidemiology of Giardia and Eimeria species in Japan: a comprehensive review.The Journal of veterinary medical science · 2024Review
- First report of Giardia duodenalis in dairy cattle and beef cattle in Shanxi, China.Molecular biology reports · 2024Article
- Dynamics of protozoal excretion in the faeces of calves during the first 28 days after arrival at the fattening farm indicate infection before regrouping and show poor temporal correlation with diarrhoea.Parasites & vectors · 2023Article
- Potential Zoonotic Transmission ofTransboundary and emerging diseases · 2023Article
- Enterocytozoon bieneusi in fecal samples from calves and cows in Austria.Parasitology research · 2023Article
- Molecular Detection and Distribution ofTransboundary and emerging diseases · 2023Article
- Diagnosis and control of cryptosporidiosis in farm animals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Population genetics of Cryptosporidium parvum subtypes in cattle in Poland: the geographical change of strain prevalence and circulation over time.BMC veterinary research · 2022Article
- High Occurrence of Zoonotic Subtypes ofMicroorganisms · 2022Article
- Literature Review: Coinfection in Young Ruminant Livestock-Pathogens (Basel, Switzerland) · 2022Review
- Prevalence and multilocus genotyping ofFrontiers in veterinary science · 2022Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To obtain information about the occurrence and genotype distribution of G. intestinalis and C. parvum in Austrian cattle, faecal samples from diarrhoeic calves younger than 180 days of age originating from 70 farms were examined. Of the 177 faecal samples, 27.1% were positive for Giardia cysts (immunofluorescence microscopy) and 55.4% for Cryptosporidium oocysts (phase-contrast microscopy). Positive samples were characterized by nested PCR for Giardia, 83.3% (triosephosphate isomerase; tpi) and 89.6% (β-giardin; bg) were positive, while the Cryptosporidium nested PCR returned 92.5% (60-kDa glycoprotein) positive results. Sequence analysis revealed one assemblage A-positive sample and 30 (bg) respectively 29 (tpi) assemblage E-positive samples for G. intestinalis. For C. parvum four subtypes within the IIa family (IIaA15G2R1, n = 29; IIaA19G2R2, n = 3; IIaA21G2R1, n = 2; IIaA14G1R1, n = 1) could be differentiated. Validation of two immunochromatographic point-of-care tests resulted in a sensitivity of 29.2% and 77.6%; a specificity of 98.4% and 91.1% for the detection of Giardia intestinalis and Cryptosporidium parvum, respectively. Results confirm the widespread occurrence of both protozoa in diarrhoeic calves in Austria.
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