ArticlePloS one2019
Mining of simple sequence repeats (SSRs) loci and development of novel transferability-across EST-SSR markers from de novo transcriptome assembly of Angelica dahurica.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Spatial Genomic Resource Reveals Molecular Insights into Key Bioactive-Metabolite Biosynthesis in EndangeredInternational journal of molecular sciences · 2022Article
- Development of 16 novel EST-SSR markers for species identification and cross-genus amplification in sambar, sika, and red deer.PloS one · 2022Article
- Impact of Genomic and Transcriptomic Resources on Apiaceae Crop Breeding Strategies.International journal of molecular sciences · 2021Review
- Article
- Constructing a Core Collection of the Medicinal PlantFrontiers in plant science · 2020Article
- Genetic Diversity and Population Structure ofFrontiers in genetics · 2020Article
- De novo assembly, transcriptome characterization, and simple sequence repeat marker development in duckweedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
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6 authors.
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Abstract
Angelica dahurica is a widely grown plant species with multiple uses, especially in the medical field. However, the frequent introduction of A. dahurica to new areas has made it difficult to distinguish between varieties. Simple sequence repeats (SSRs) detected based on transcriptome analyses are very useful for constructing genetic maps and analyzing genetic diversity. They are also relevant for the molecular marker-assisted breeding of A. dahurica. We identified 33,724 genic SSR loci based on transcriptome sequencing data. A total of 114 primer pairs were designed for the SSR loci and were tested for their specificity and diversity. Ten SSR loci in untranslated regions were ultimately selected. Subsequently, 56 A. dahurica ecotypes collected from different regions were analyzed. The SSR loci comprised 2-8 alleles, with a mean of 5.2 alleles per locus. The polymorphic information content value and Shannon's information index were 0.6274-0.2702 (average of 0.4091) and 1.3040-0.5618 (average of 0.8475), respectively. Thus, the 10 novel SSRs identified in this study were almost in accordance with Harvey-Weinberg equilibrium and will be useful for analyzing A. dahurica genetic relationships. The results of this study confirm the potential value of transcriptome databases for the development of new SSR markers.
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