ArticlePloS one2019
Gene expression of Porphyromonas gingivalis ATCC 33277 when growing in an in vitro multispecies biofilm.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
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- Mitochondria: An Emerging Unavoidable Link in the Pathogenesis of Periodontitis Caused byInternational journal of molecular sciences · 2024Review
- Modulatory Mechanisms of Pathogenicity inMicroorganisms · 2022Review
- Engineering peptide-polymer hybrids for targeted repair and protection of cervical lesions.Frontiers in dental medicine · 2022Article
- Review
- Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems.Microorganisms · 2021Review
- Scanning electrochemical microscopy and its potential for studying biofilms and antimicrobial coatings.Analytical and bioanalytical chemistry · 2020Review
- Role of Superoxide Reductase FA796 in Oxidative Stress Resistance in Filifactor alocis.Scientific reports · 2020Article
- PgRsp Is a Novel Redox-Sensing Transcription Regulator Essential forMicroorganisms · 2019Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivePorphyromonas gingivalis, an oral microorganism residing in the subgingival biofilm, may exert diverse pathogenicity depending on the presence of specific virulence factors, but its gene expression has not been completely established. This investigation aims to compare the transcriptomic profile of this pathogen when growing within an in vitro multispecies biofilm or in a planktonic state. MATERIALS AND
methodsP. gingivalis ATCC 33277 was grown in anaerobiosis within multi-well culture plates at 37°C under two conditions: (a) planktonic samples (no hydroxyapatite discs) or (b) within a multispecies-biofilm containing Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans deposited on hydroxyapatite discs. Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) combined with Fluorescence In Situ Hybridization (FISH) were used to verify the formation of the biofilm and the presence of P. gingivalis. Total RNA was extracted from both the multispecies biofilm and planktonic samples, then purified and, with the use of a microarray, its differential gene expression was analyzed. A linear model was used for determining the differentially expressed genes using a filtering criterion of two-fold change (up or down) and a significance p-value of <0.05. Differential expression was confirmed by Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR).
resultsSEM verified the development of the multispecies biofilm and FISH confirmed the incorporation of P. gingivalis. The microarray demonstrated that, when growing within the multispecies biofilm, 19.1% of P. gingivalis genes were significantly and differentially expressed (165 genes were up-regulated and 200 down-regulated), compared with planktonic growth. These genes were mainly involved in functions related to the oxidative stress, cell envelope, transposons and metabolism. The results of the microarray were confirmed by RT-qPCR.
conclusionSignificant transcriptional changes occurred in P. gingivalis when growing in a multispecies biofilm compared to planktonic state.
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