Evidence map›Paper›PMID 31436030›Full record

ArticleMicrobiologyOpen2019

Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae.

Christoph Rieck, Daniel Geiger, Jennifer Munkert, Katrin Messerschmidt, Jan Petersen, Juliane Strasser, Nadine Meitinger, Wolfgang Kreis

Abstract read
In one paragraph

Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Characterization of two key cytochrome P450 enzymes inSynthetic and systems biotechnology · 2027
    Article
  2. Article
  3. Knockout ofMetabolites · 2021
    Article
  4. Article
  5. Toxins · 2021
    Review
  6. Yeast as a promising heterologous host for steroid bioproduction.Journal of industrial microbiology & biotechnology · 2020
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Christoph RieckDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.ORCID 0000-0002-2746-998X
Daniel GeigerDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Jennifer MunkertDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Katrin MesserschmidtCell2Fab Research Unit, University of Potsdam, Potsdam, Germany.
Jan PetersenDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Juliane StrasserDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Nadine MeitingerDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Wolfgang KreisDepartment Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ

Indexed as

Biosynthetic PathwaysMetabolic EngineeringCardenolidesGas Chromatography-Mass SpectrometryGene Expression Regulation, EnzymologicGene Expression Regulation, FungalGene OrderPlasmidsSaccharomyces cerevisiaeCardenolidesbaker's yeastbiosynthesiscardenolidespathway engineeringsteroids

Identifiers

PMID31436030
PMCPMC6925150

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.