ArticleAMB Express2019
Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius.
Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- The Phenylpropanoid Gatekeeper CtPAL1 Coordinates ABA-Induced Flavonoid Biosynthesis and Oxidative Stress Tolerance in Safflower (Plants (Basel, Switzerland) · 2025Article
- Research Progress of Genomics Applications in Secondary Metabolites of Medicinal Plants: A Case Study in Safflower.International journal of molecular sciences · 2025Review
- Bioinformatics and Expression Analysis of CHI Gene Family in Sweet Potato.Plants (Basel, Switzerland) · 2025Article
- Safflower CtFT genes orchestrating flowering time and flavonoid biosynthesis.BMC plant biology · 2024Article
- Identification of ANS gene regulating fruit color in jujube (Ziziphus jujuba Mill. cv. Lingwuchangzao) and analysis of its expression under elevated temperature and drought stress.BMC plant biology · 2024Article
- Integrated Metabolomics and Transcriptomics Provide Key Molecular Insights into Floral Stage-Driven Flavonoid Pathway in Safflower.International journal of molecular sciences · 2024Article
- SafflowerInternational journal of molecular sciences · 2024Article
- Article
- The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L.BMC plant biology · 2023Article
- High interspecific competitiveness of the invasive plant Xanthium italicum Moretti severely reduces the yield and quality of Carthamus tinctorius L.Scientific reports · 2023Article
- Molecular Characterization of anMolecules (Basel, Switzerland) · 2022Article
- Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region onSynthetic and systems biotechnology · 2022Article
- Genome-wide analysis and transcriptional reprogrammings of MYB superfamily revealed positive insights into abiotic stress responses and anthocyanin accumulation in Carthamus tinctorius L.Molecular genetics and genomics : MGG · 2022Article
- Multi-Omics and miRNA Interaction Joint Analysis Highlight New Insights Into Anthocyanin Biosynthesis in Peanuts (Frontiers in plant science · 2022Article
- Adaptive Evolution of Chalcone Isomerase Superfamily in Fagaceae.Biochemical genetics · 2021Article
- Identification and Characterization of Chalcone Isomerase Genes Involved in Flavonoid Production inFrontiers in plant science · 2021Article
- Anthocyanins in Floral Colors: Biosynthesis and Regulation in Chrysanthemum Flowers.International journal of molecular sciences · 2020Review
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Authors and funding
13 authors at 1 institution in 1 country.
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Abstract
Flavonoid is one of the widespread groups of plant secondary metabolites that provide several health benefits. However, the explicit mechanism of flavonoid biosynthesis in plants largely remains unclear. Chalcone isomerase an important class of enzyme presents crucial role during flavonoid metabolism in many plants. Here, we isolated the full-length cDNA (1161 bp) of a novel Chalcone Isomerase from safflower encoding 217 amino acid polypeptide using oligos from 5' and 3' ends. The result of Sanger sequencing and phylogenetic analysis revealed that CtCHI is highly homologous to other plants, including typical polyadenylation signals AATAA and Poly A tail. The transient expression in tobacco mesophyll cells using Green Fluorescent Protein tagging determined the subcellular localization of CtCHI in cell membrane and nucleus. The CtCHI ectopic expression in different safflower varieties at different flowering stages showed that CtCHI were found in abundance at the bud stage of Jihong No. 1. Further correlation analysis between CtCHI expression and flavonoid accumulation at various flowering phases suggested that CtCHI might play a potential role during flavonoid biosynthesis in safflower. In addition, the overexpression of pBASTA-CtCHI in transgenic Arabidopsis infiltrated with floral dip transformation showed relatively higher expression level and increased flavonoid accumulation than wild type. Moreover, the in vitro enzymatic activity and HPLC analysis of transgenic Arabidopsis confirmed the de novo biosynthesis of Rutin. Taken together, our findings laid the foundation of identifying an important gene that might influence flavonoid metabolism in safflower.
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