Evidence map›Paper›PMID 31435686›Full record

ArticleAnalytical and bioanalytical chemistry2019

Novel probes for label-free detection of neurodegenerative GGGGCC repeats associated with amyotrophic lateral sclerosis.

Motahareh Taki, Kushal J Rohilla, Maria Barton, Madison Funneman, Najiyah Benzabeh, Swati Naphade, Lisa M Ellerby, Keith T Gagnon, Mohtashim H Shamsi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Motahareh TakiDepartment of Chemistry & Biochemistry, Southern Illinois University, 1245 Lincoln Dr, Carbondale, IL, 62901, USA.
Kushal J RohillaBiochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL, 62901, USA.
Maria BartonBiochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL, 62901, USA.
Madison FunnemanDepartment of Chemistry & Biochemistry, Southern Illinois University, 1245 Lincoln Dr, Carbondale, IL, 62901, USA.
Najiyah BenzabehDepartment of Chemistry & Biochemistry, Southern Illinois University, 1245 Lincoln Dr, Carbondale, IL, 62901, USA.
Swati NaphadeThe Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA, 94945, USA.
Lisa M EllerbyThe Buck Institute for Research on Aging, 8001 Redwood Blvd, Novato, CA, 94945, USA.
Keith T GagnonDepartment of Chemistry & Biochemistry, Southern Illinois University, 1245 Lincoln Dr, Carbondale, IL, 62901, USA.
Mohtashim H ShamsiDepartment of Chemistry & Biochemistry, Southern Illinois University, 1245 Lincoln Dr, Carbondale, IL, 62901, USA. mshamsi@siu.edu.ORCID http://orcid.org/0000-0002-5463-811X
Southern Illinois University Carbondale · USBuck Institute for Research on Aging · USSouthern Illinois University School of Medicine · US

Funding

Identifying Factors Regulating Medium Spiny Neuron Differentiation or Maintenance as Therapeutic Targets for Huntington's Disease using Induced Pluripotent Stem CellsR01NS100529 · NINDS · BUCK INSTITUTE FOR RESEARCH ON AGING · PI Lisa M Ellerby · 2016 to 2026
$5.6M
Nuclear Surveillance of Expanded Tandem Repeat RNA in C9FTD/ALS DiseaseR15NS111374 · NINDS · SOUTHERN ILLINOIS UNIVERSITY CARBONDALE · PI GAGNON, KEITH THOMAS · 2019 to 2019
$443k
NIH HHS R01 NS100529NINDS NIH HHS R01 NS100529NINDS NIH HHS R15 NS111374
6 · The paper itself

Abstract

DNA repeat expansion sequences cause a myriad of neurological diseases when they expand beyond a critical threshold. Previous electrochemical approaches focused on the detection of trinucleotide repeats (CAG, CGG, and GAA) and relied on labeling of the probe and/or target strands or enzyme-linked assays. However, detection of expanded GC-rich sequences is challenging because they are prone to forming secondary structures such as cruciforms and quadruplexes. Here, we present label-free detection of hexanucleotide GGGGCC repeat sequences, which cause the leading genetic form of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). The approach relies on capturing targets by surface-bound oligonucleotide probes with a different number of complementary repeats, which proportionately translates the length of the target strands into charge transfer resistance (R

Indexed as

DNA Repeat ExpansionAmyotrophic Lateral SclerosisBase SequenceBiosensing TechniquesDielectric SpectroscopyHumansHuntington DiseaseOligonucleotide ProbesRNAOligonucleotide ProbesRNAAmyotrophic lateral sclerosisElectrochemical biosensorsElectrochemical impedance spectroscopyNucleic acid biosensingRepeat expansion disorders

Identifiers

PMID31435686
PMCPMC7433021
OpenAlexW2969593912

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.