Evidence map›Paper›PMID 31434319›Full record

ArticleVaccines2019

Attenuation of Zika Virus by Passage in Human HeLa Cells.

Li Li, Natalie D Collins, Steven G Widen, Emily H Davis, Jaclyn A Kaiser, Mellodee M White, M Banks Greenberg, Alan D T Barrett, Nigel Bourne, Vanessa V Sarathy

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccines, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Zika virus infection in the genital tract of non-pregnant females: a systematic review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Li LiDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Natalie D CollinsDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Steven G WidenDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Emily H DavisDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Jaclyn A KaiserDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Mellodee M WhiteDepartment of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555, USA.
M Banks GreenbergDepartment of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555, USA.
Alan D T BarrettDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Nigel BourneDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Vanessa V SarathyDepartment of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA. vvsarath@utmb.edu.
The University of Texas Medical Branch at Galveston · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zika virus (ZIKV) is a mosquito-borne Flavivirus. Previous studies have shown that mosquito-transmitted flaviviruses, including yellow fever, Japanese encephalitis, and West Nile viruses, could be attenuated by serial passaging in human HeLa cells. Therefore, it was hypothesized that wild-type ZIKV would also be attenuated after HeLa cell passaging. A human isolate from the recent ZIKV epidemic was subjected to serial HeLa cell passaging, resulting in attenuated in vitro replication in both Vero and A549 cells. Additionally, infection of AG129 mice with 10 plaque forming units (pfu) of wild-type ZIKV led to viremia and mortality at 12 days, whereas infection with 10

Indexed as

attenuationflavivirusHeLa cellsZika virusZika virus NS1Zika virus prM

Identifiers

PMID31434319
PMCPMC6789458
OpenAlexW2969599686

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.