Evidence map›Paper›PMID 31432636›Full record

ArticleAnnals of laboratory medicine2020

A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of

Naoki Kakuta, Ryuichi Nakano, Akiyo Nakano, Yuki Suzuki, Ayako Tanouchi, Takashi Masui, Saori Horiuchi, Shiro Endo, Risako Kakuta, Yasuo Ono and 1 more

Open access · diamondAbstract read
In one paragraph

Article in Annals of laboratory medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.3field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

  1. Virulence · 2026
    Review
  2. The intricacies ofFrontiers in microbiology · 2025
    Review
  3. Article
  4. Review
  5. Molecular detection of fluoroquinolone-resistantFrontiers in cellular and infection microbiology · 2022
    Article
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Naoki Kakuta *Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0001-9508-577X
Ryuichi Nakano *Department of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan. rnakano@naramed-u.ac.jp.ORCID https://orcid.org/0000-0003-2086-2591
Akiyo NakanoDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0003-1379-384X
Yuki SuzukiDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0002-2509-8513
Ayako TanouchiDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0002-6096-7630
Takashi MasuiDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0002-9567-2947
Saori HoriuchiDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0002-4143-5807
Shiro EndoInternational University of Health and Welfare, Shioya Hospital, Tochigi, Japan.ORCID https://orcid.org/0000-0003-0103-415X
Risako KakutaDepartment of Otolaryngology-Head and Neck Surgery, Tohoku University Graduate School of Medicine, Miyagi, Japan.ORCID https://orcid.org/0000-0002-4594-908X
Yasuo OnoDepartment of Microbiology and Immunology, Teikyo University School of Medicine, Tokyo, Japan.ORCID https://orcid.org/0000-0002-5211-8102
Hisakazu YanoDepartment of Microbiology and Infectious Diseases, Nara Medical University, Nara, Japan.ORCID https://orcid.org/0000-0003-2085-7194
Nara Medical University · JPInternational University of Health and Welfare · JPTeikyo University · JPTohoku University · JP

Funding

Japan Society for the Promotion of Science 17K10027Japan Society for the Promotion of Science 18K09935
6 · The paper itself

Abstract

backgroundMutations in the quinolone resistance-determining regions (QRDRs) of

methodsBased on the conserved sequences of

resultsPCR amplification of

conclusionsThis assay specifically amplified

Indexed as

Acinetobacter baumanniiAnti-Bacterial AgentsBase Pair MismatchDNA, BacterialDNA GyraseDNA Topoisomerase IVDrug Resistance, BacterialFluoroquinolonesMicrobial Sensitivity TestsMutationPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSequence Analysis, DNAAnti-Bacterial AgentsDNA, BacterialDNA GyraseDNA Topoisomerase IVFluoroquinolonesAcinetobacter baumanniiFluoroquinolone resistancePCR-restriction fragment length polymorphismQuinolone resistance-determining regions

Identifiers

PMID31432636
PMCPMC6713654
OpenAlexW2969884783

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.