Evidence map›Paper›PMID 31430902›Full record

ReviewInternational journal of molecular sciences2019

Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox.

Ali Razzaq, Fozia Saleem, Mehak Kanwal, Ghulam Mustafa, Sumaira Yousaf, Hafiz Muhammad Imran Arshad, Muhammad Khalid Hameed, Muhammad Sarwar Khan, Faiz Ahmad Joyia

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed.

  1. Review
  2. Review
  3. From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026
    Review
  4. Review
  5. Review
  6. Molecular phylogenetics and evolutionary history ofFrontiers in plant science · 2026
    Review
  7. Review
  8. Agrobacterium tumefaciens-Mediated Genome Editing in Banana.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Rising from the dead: the power of genome editing.Science China. Life sciences · 2023
    Article
  14. Plant Genomics-Advancing Our Understanding of Plants.International journal of molecular sciences · 2023
    Article
  15. Review
  16. Article
  17. Review
  18. CRISPR/Cas9-mediated editing ofFrontiers in plant science · 2023
    Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ali RazzaqCentre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan.ORCID 0000-0002-9122-3714
Fozia SaleemCentre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan.
Mehak KanwalNuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Faisalabad 38000, Pakistan.
Ghulam MustafaCentre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan.
Sumaira YousafNuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Faisalabad 38000, Pakistan.
Hafiz Muhammad Imran ArshadNuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Faisalabad 38000, Pakistan.
Muhammad Khalid HameedSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Muhammad Sarwar KhanCentre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan.ORCID 0000-0001-7194-8622
Faiz Ahmad JoyiaCentre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan. faizahmad1980@uaf.edu.pk.ORCID 0000-0002-9540-6972

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increasing agricultural productivity via modern breeding strategies is of prime interest to attain global food security. An array of biotic and abiotic stressors affect productivity as well as the quality of crop plants, and it is a primary need to develop crops with improved adaptability, high productivity, and resilience against these biotic/abiotic stressors. Conventional approaches to genetic engineering involve tedious procedures. State-of-the-art OMICS approaches reinforced with next-generation sequencing and the latest developments in genome editing tools have paved the way for targeted mutagenesis, opening new horizons for precise genome engineering. Various genome editing tools such as transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and meganucleases (MNs) have enabled plant scientists to manipulate desired genes in crop plants. However, these approaches are expensive and laborious involving complex procedures for successful editing. Conversely, CRISPR/Cas9 is an entrancing, easy-to-design, cost-effective, and versatile tool for precise and efficient plant genome editing. In recent years, the CRISPR/Cas9 system has emerged as a powerful tool for targeted mutagenesis, including single base substitution, multiplex gene editing, gene knockouts, and regulation of gene transcription in plants. Thus, CRISPR/Cas9-based genome editing has demonstrated great potential for crop improvement but regulation of genome-edited crops is still in its infancy. Here, we extensively reviewed the availability of CRISPR/Cas9 genome editing tools for plant biotechnologists to target desired genes and its vast applications in crop breeding research.

Indexed as

CRISPR-Cas SystemsGenome, PlantCrops, AgriculturalGene EditingPlant BreedingPlantsPlants, Genetically Modifiedabiotic stressbiotic stressCRISPR/Cas9crop improvementgenome editingmultiplex genome editingplant breedingTALENZFN

Identifiers

PMID31430902
PMCPMC6720679

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.