Evidence map›Paper›PMID 31430893›Full record

ArticleInternational journal of molecular sciences2019

Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence Are Damaged by In Vitro Exposure to its Dyslipidemic Serum, Predicting Inadequate Repair Capacity In Vivo.

Istvan Kovanecz, Robert Gelfand, Guiting Lin, Sheila Sharifzad, Alec Ohanian, Randy Ricks, Tom Lue, Nestor F Gonzalez-Cadavid

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
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  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Istvan KovaneczDivision of Urology, Department of Surgery, Harbor-UCLA Medical Center and Los Angeles Biomedical Research Institute, Torrance, 90502 CA, USA.ORCID 0000-0002-3514-2338
Robert GelfandDivision of Urology, Department of Surgery, Harbor-UCLA Medical Center and Los Angeles Biomedical Research Institute, Torrance, 90502 CA, USA.
Guiting LinDepartment of Urology, UCSF School of Medicine, San Francisco, 94143 CA, USA.
Sheila SharifzadDivision of Urology, Department of Surgery, Harbor-UCLA Medical Center and Los Angeles Biomedical Research Institute, Torrance, 90502 CA, USA.
Alec OhanianDivision of Urology, Department of Surgery, Harbor-UCLA Medical Center and Los Angeles Biomedical Research Institute, Torrance, 90502 CA, USA.ORCID 0000-0003-0279-7298
Randy RicksDepartment of Medicine, Charles Drew University of Medicine and Science, Los Angeles, 90059 CA, USA.
Tom LueDepartment of Urology, UCSF School of Medicine, San Francisco, 94143 CA, USA.
Nestor F Gonzalez-CadavidDivision of Urology, Department of Surgery, Harbor-UCLA Medical Center and Los Angeles Biomedical Research Institute, Torrance, 90502 CA, USA. ncadavid@ucla.edu.ORCID 0000-0003-3181-2944
The Lundquist Institute · USUniversity of California, Los Angeles · USUniversity of California, San Francisco · USCharles R. Drew University of Medicine and Science · US

Funding

Therapy for Obesity-associated Stress Urinary IncontinenceR01DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2016 to 2019
$2.6M
LABioMed Research Institute at Harbor-UCLA Medical Center 531840-01-00NIDDK NIH HHS R01 DK105097NIH HHS 1R01DK105097
6 · The paper itself

Abstract

Female stress urinary incontinence (FSUI) is prevalent in women with type 2 diabetes/obesity (T2D/O), and treatment is not optimal. Autograph stem cell therapy surprisingly has poor efficacy. In the male rat model of T2D/O, it was demonstrated that epigenetic changes, triggered by long-term exposure to the dyslipidemic milieu, led to abnormal global transcriptional signatures (GTS) of genes and microRNAs (miR), and impaired the repair capacity of muscle-derived stem cells (MDSC). This was mimicked in vitro by treatment of MDSC with dyslipidemic serum or lipid factors. The current study aimed to predict whether these changes also occur in stem cells from female 12 weeks old T2D/O rats, a model of FSUI. MDSCs from T2D/O (ZF4-SC) and normal female rats (ZL4-SC) were treated in vitro with either dyslipidemic serum (ZFS) from late T2D/O 24 weeks old female Zucker fatty (ZF) rats, or normal serum (ZLS) from 24 weeks old female Zucker lean (ZL) rats, for 4 days and subjected to assays for fat deposition, apoptosis, scratch closing, myostatin, interleukin-6, and miR-GTS. The dyslipidemic ZFS affected both female stem cells more severely than in the male MDSC, with some gender-specific differences in miR-GTS. The changes in miR-GTS and myostatin/interleukin-6 balance may predict in vivo noxious effects of the T2D/O milieu that might impair autograft stem cell (SC) therapy for FSUI, but this requires future studies.

Indexed as

AnimalsApoptosisCells, CulturedDiabetes Mellitus, Type 2Disease Models, AnimalDyslipidemiasFemaleMaleRatsRats, ZuckerStem CellsStem Cell TransplantationUrinary Incontinenceapoptosisdyslipidemiafat infiltrationinterleukin-6microRNAmuscle-derived stem cellsmyostatinwound closure

Identifiers

PMID31430893
PMCPMC6720976
OpenAlexW2968116521

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.