ArticleBritish journal of pharmacology2019
Characterisation of nerve-mediated ATP release from bladder detrusor muscle and its pathological implications.
Article in British journal of pharmacology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Parasympathetic overdrive as a cause of overactive bladder in a high-fat-diet-induced obesity mouse model.The Journal of physiology · 2026Article
- Selection of sciatic nerve injury models: implications for pathogenesis and treatment.Frontiers in neurology · 2025Review
- Role of ecto-5'-nucleotidase in bladder function activity and smooth muscle contractility.Purinergic signalling · 2024Article
- New therapeutic targets to prevent benign prostatic enlargement and symptomatic progression to benign prostatic obstruction-ICI-RS 2023.Neurourology and urodynamics · 2024Review
- Excitatory purinergic and cholinergic expression changed in a partial bladder outlet obstruction-induced overactive bladder rat model.Scientific reports · 2023Article
- Targeting neurotrophin and nitric oxide signaling to promote recovery and ameliorate neurogenic bladder dysfunction following spinal cord injury - Mechanistic concepts and clinical implications.Continence (Amsterdam, Netherlands) · 2023Article
- Soluble Guanylate Cyclase Activators to Treat Benign Prostatic Hyperplasia and associated LUTS.Continence (Amsterdam, Netherlands) · 2023Article
- Selective reduction of neurotransmitter release by cAMP-dependent pathways in mouse detrusor.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- Role of PTHrP in attenuating transient pressure rises and associated afferent nerve activity of the rat bladder.Pflugers Archiv : European journal of physiology · 2022Article
- Frequency-dependent characteristics of nerve-mediated ATP and acetylcholine release from detrusor smooth muscle.Experimental physiology · 2022Article
- Purinergic signalling in the urinary bladder - When function becomes dysfunction.Autonomic neuroscience : basic & clinical · 2021Article
- The Frequency-Dependence of Pre- and Postganglionic Nerve Stimulation of Pig and Rat Bladder.International neurourology journal · 2021Article
- High CD39 expression is associated with the non-muscle-invasive phenotype of human bladder cancer.Oncotarget · 2021Article
- Neurophysiological control of urinary bladder storage and voiding-functional changes through development and pathology.Pediatric nephrology (Berlin, Germany) · 2021Review
- Low-Dose Cyclophosphamide Induces Nerve Injury and Functional Overactivity in the Urinary Bladder of Rats.Frontiers in neuroscience · 2021Article
- Characterisation of nerve-mediated ATP release from bladder detrusor muscle and its pathological implications.British journal of pharmacology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
background and purposeThis study aims to characterise the molecular mechanisms that determine variability of atropine resistance of nerve-mediated contractions in human and guinea pig detrusor smooth muscle. EXPERIMENTAL APPROACH: Atropine resistance of nerve-mediated contractions and the role of P2X1 receptors, were assessed in isolated preparations from guinea pigs and also humans with or without overactive bladder syndrome, from which the mucosa was removed. Nerve-mediated ATP release was measured directly with amperometric ATP-sensitive electrodes. Ecto-ATPase activity of guinea pig and human detrusor samples was measured in vitro by measuring the concentration-dependent rate of ATP breakdown. The transcription of ecto-ATPase subtypes in human samples was measured by qPCR. KEY
resultsAtropine resistance was greatest in guinea pig detrusor, absent in human tissue from normally functioning bladders, and intermediate in human overactive bladder. Greater atropine resistance correlated with reduction of contractions by the ATP-diphosphohydrolase apyrase, directly implicating ATP in their generation. E-NTPDase-1 was the most abundantly transcribed ecto-ATPase of those tested, and transcription was reduced in tissue from human overactive, compared to normal, bladders. E-NTPDase-1 enzymic activity was inversely related to the magnitude of atropine resistance. Nerve-mediated ATP release was continually measured and varied with stimulation frequency over the range of 1-16 Hz. CONCLUSION AND IMPLICATIONS: Atropine resistance in nerve-mediated detrusor contractions is due to ATP release and its magnitude is inversely related to E-NTPDase-1 activity. ATP is released under different stimulation conditions compared with ACh, implying different routes for their release.
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