ArticleBiotechnology and bioengineering2019
Direct electrical stimulation enhances osteogenesis by inducing Bmp2 and Spp1 expressions from macrophages and preosteoblasts.
Article in Biotechnology and bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Biodegradable molybdenum intramedullary nails facilitate fracture repair through neutrophil-mediated immune response.Materials today. Bio · 2026Article
- Electrical stimulation as an emerging strategy in bone repair: Mechanisms, applications, and advances.Journal of orthopaedic translation · 2026Review
- The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair.Stem cell reports · 2026Article
- Advanced multifunctional thermo- and electro-stimulative hydrogels for bone regeneration.Materials today. Bio · 2026Review
- Clinical Applications of Biophysical Stimuli Technologies for Bone Healing.Annals of biomedical engineering · 2026Review
- Degradable piezoelectric KNN/PLLA nanofibers for promoting osteogenesis and angiogenesis in bone regeneration.Biomedical engineering online · 2026Article
- In vitro electrical stimulation devices: practical framework for design, fabrication, and operation.Journal of biological engineering · 2026Article
- Harnessing Piezoelectric Biomaterials for Pathogenic Eradication and Tissue Regeneration.Exploration (Beijing, China) · 2026Review
- The Bionic Interface: Considering the Material Mediated Electrical Stimulation of Stem Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- The crucial role of SPP1 in osteoporosis, osteoarthritis, and cancer.Pharmaceutical science advances · 2025Review
- Open Challenges and Opportunities in Piezoelectricity for Tissue Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Review
- Accelerated Bone Healing via Electrical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Electro-spun piezoelectric PLLA smart composites as a scaffold on bone fracture: A review.Regenerative therapy · 2025Review
- From innovation to clinic: Emerging strategies harnessing electrically conductive polymers to enhance electrically stimulated peripheral nerve repair.Materials today. Bio · 2025Article
- Small extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity.BMC veterinary research · 2025Article
- The effect of barium titanate-coated titanium alloy scaffolds on bone regeneration in osteonecrosis of the femoral head models: a comprehensive analysis based onFrontiers in bioengineering and biotechnology · 2025Article
- Electroactive electrospun nanoplatform combined with electrical stimulation modulates anti-inflammatory macrophage polarization for enhanced wound healing.Frontiers in bioengineering and biotechnology · 2025Article
- Activation of the pentose phosphate pathway by microcurrent stimulation mediates antioxidant effects in inflammation-stimulated macrophages.Frontiers in physiology · 2025Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The capability of electrical stimulation (ES) in promoting bone regeneration has already been addressed in clinical studies. However, its mechanism is still being investigated and discussed. This study aims to investigate the responses of macrophages (J774A.1) and preosteoblasts (MC3T3-E1) to ES and the faradic by-products from ES. It is found that pH of the culture media was not significantly changed, whereas the average hydrogen peroxide concentration was increased by 3.6 and 5.4 µM after 1 and 2 hr of ES, respectively. The upregulation of Bmp2 and Spp1 messenger RNAs was observed after 3 days of stimulation, which is consistent among two cell types. It is also found that Spp1 expression of macrophages was partially enhanced by faradic by-products. Osteogenic differentiation of preosteoblasts was not observed during the early stage of ES as the level of Runx2 expression remains unchanged. However, cell proliferation was impaired by the excessive current density from the electrodes, and also faradic by-products in the case of macrophages. This study shows that macrophages could respond to ES and potentially contribute to the bone formation alongside preosteoblasts. The upregulation of Bmp2 and Spp1 expressions induced by ES could be one of the mechanisms behind the electrically stimulated osteogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.