Improving ionic liquid tolerance in Saccharomyces cerevisiae through heterologous expression and directed evolution of an ILT1 homolog from Yarrowia lipolytica.

Kevin B Reed, James M Wagner, Simon d'Oelsnitz, Joshua M Wiggers, Hal S Alper

Abstract read

In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it

0cells of the map it votes in

4citing papers in PubMed

1.4field-weighted citation impact, top 19% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 22 citations in OpenAlex.

Ciprofloxacin-Based Ionic Liquids Increase Mutation Frequency inAntibiotics (Basel, Switzerland) · 2026
Article
A role for ion homeostasis in yeast ionic liquid tolerance.microPublication biology · 2023
Article
Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolytica.mSystems · 2022
Article
Ethanol stress responses in Kluyveromyces marxianus: current knowledge and perspectives.Applied microbiology and biotechnology · 2022
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kevin B ReedMcKetta Department of Chemical Engineering, The University of Texas at Austin, 200 East Dean Keeton St., Stop C0400, Austin, TX, 78712, USA.

James M WagnerMcKetta Department of Chemical Engineering, The University of Texas at Austin, 200 East Dean Keeton St., Stop C0400, Austin, TX, 78712, USA.

Simon d'OelsnitzInstitute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX, 78712, USA.

Joshua M WiggersMcKetta Department of Chemical Engineering, The University of Texas at Austin, 200 East Dean Keeton St., Stop C0400, Austin, TX, 78712, USA.

Hal S AlperMcKetta Department of Chemical Engineering, The University of Texas at Austin, 200 East Dean Keeton St., Stop C0400, Austin, TX, 78712, USA. halper@che.utexas.edu.ORCID http://orcid.org/0000-0002-8246-8605

The University of Texas at Austin · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ionic liquids show promise for deconstruction of lignocellulosic biomass prior to fermentation. Yet, imidazolium ionic liquids (IILs) can be toxic to microbes even at concentrations present after recovery. Here, we show that dominant overexpression of an Ilt1p homolog (encoded by YlILT1/YALI0C04884) from the IIL-tolerant yeast Yarrowia lipolytica confers an improvement in 1-ethyl-3-methylimidazolium acetate tolerance in Saccharomyces cerevisiae compared to the endogenous Ilt1p (ScILT1/YDR090C). We subsequently enhance tolerance in S. cerevisiae through directed evolution of YlILT1 using growth-based selection, leading to identification of mutants that grow in up to 3.5% v/v ionic liquid. Lastly, we demonstrate that strains expressing YlILT1 variants demonstrate improved growth rate and ethanol production in the presence of residual IIL. This shows that dominant overexpression of a heterologous protein (wild type or evolved) from an IIL-tolerant yeast can increase tolerance in S. cerevisiae at concentrations relevant to bioethanol production from IIL-treated biomass.

Indexed as

BiomassEthanolEvolution, MolecularFermentationGene Expression Regulation, FungalImidazolesIonic LiquidsSaccharomyces cerevisiaeYarrowia1-ethyl-3-methylimidazoliumEthanolImidazolesIonic LiquidsDirected evolutionImidazolium ionic liquid toleranceIonic liquidsSaccharomyces cerevisiaeYarrowia lipolytica

Identifiers

PMID31428944

DOI10.1007/s10295-019-02228-9

OpenAlexW2969385236

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.