ArticleNucleic acids research2019
DNA polymerase activity on synthetic N3'→P5' phosphoramidate DNA templates.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A Facile Strategy for 3'-Terminal Functionalization of DNA and RNA via On-Column Conjugation with Integration into Tandem Oligonucleotide Synthesis.Current protocols · 2026Article
- cGAS-STING pathway in lung cancer and emerging therapeutic approaches.Drug discovery today · 2026Review
- Derivatization of Solid Supports Using a Carbamate-Linked Tether Containing a Disulfide Functional Group.Current protocols · 2025Article
- A Versatile Disulfide-Containing Solid-Support Strategy for 3'-Modifiers in Oligonucleotides: Introducing Modular Tandem Oligonucleotide Synthesis.Chemistry, an Asian journal · 2025Article
- Trivalent rare earth metal cofactors confer rapid NP-DNA polymerase activity.Science (New York, N.Y.) · 2023Article
- Introducing a New Bond-Forming Activity in an Archaeal DNA Polymerase by Structure-Guided Enzyme Redesign.ACS chemical biology · 2022Article
- Synthetic Life with Alternative Nucleic Acids as Genetic Materials.Molecules (Basel, Switzerland) · 2020Review
- Deep sequencing of non-enzymatic RNA primer extension.Nucleic acids research · 2020Article
- Synthesis of phosphoramidate-linked DNA by a modified DNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Stereoselective Syntheses of 3'-Hydroxyamino- and 3'-Methoxyamino-2',3'-Dideoxynucleosides.Organic letters · 2019Article
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Authors and funding
5 authors.
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Abstract
Genetic polymers that could plausibly govern life in the universe might inhabit a broad swath of chemical space. A subset of these genetic systems can exchange information with RNA and DNA and could therefore form the basis for model protocells in the laboratory. N3'→P5' phosphoramidate (NP) DNA is defined by a conservative linkage substitution and has shown promise as a protocellular genetic material, but much remains unknown about its functionality and fidelity due to limited enzymatic tools. Conveniently, we find widespread NP-DNA-dependent DNA polymerase activity among reverse transcriptases, an observation consistent with structural studies of the RNA-like conformation of NP-DNA duplexes. Here, we analyze the consequences of this unnatural template linkage on the kinetics and fidelity of DNA polymerization activity catalyzed by wild-type and variant reverse transcriptases. Template-associated deficits in kinetics and fidelity suggest that even highly conservative template modifications give rise to error-prone DNA polymerase activity. Enzymatic copying of NP-DNA sequences is nevertheless an important step toward the future study and engineering of this synthetic genetic polymer.
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