Evidence map›Paper›PMID 31427900›Full record

ArticleBiological procedures online2019

Genetic Manipulation of MicroRNAs in the Silk Gland of Silkworm,

Wei Wang, Xinran Wang, Xuemei Li, Qian Pu, Chengyi Luo, Lili Xu, Xinyue Peng, Shiping Liu

Abstract read
In one paragraph

Article in Biological procedures online, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. ame-miR-5119-Frontiers in physiology · 2024
    Article
  3. ame-miR-34 Modulates the Larval Body Weight and Immune Response ofInternational journal of molecular sciences · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wei Wang1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Xinran Wang1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Xuemei Li1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Qian Pu1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Chengyi Luo1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Lili Xu1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Xinyue Peng1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.
Shiping Liu1State Key Laboratory of Silkworm Genome Biology,Biological Science Research Center, Southwest University, Chongqing, 400715 People's Republic of China.ORCID 0000-0002-2917-0172

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroRNAs (miRNAs) are a class of non-coding RNAs with important post-transcriptional regulatory functions. To reveal the function of miRNAs

resultsInjection of synthesized microRNA mimics and antagomirs exerted no effect on the expression of miR-274 in the silk gland, miR-274 sponge specifically absorbed miR-274 and down-regulated its expression, transgenic overexpression of miR-274 precursor significantly up-regulated miR-274, and finally tissue-specific CRISPR/Cas9 system achieved deletion of miR-274.

conclusionsA practical technical system was established for studying the functions of miRNAs in silk gland of

Indexed as

Bombyx moriCRISPR/Cas9Functional studyKnockoutmicroRNASilk gland

Identifiers

PMID31427900
PMCPMC6694536

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.