ArticleNature chemistry2019
Building in vitro transcriptional regulatory networks by successively integrating multiple functional circuit modules.
Article in Nature chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Programmable DNA-Based Dimerization Networks for Dynamic and Competitive Control of Transcription.Journal of the American Chemical Society · 2026Article
- A DNA-encoded recipe to direct multistage colloidal assembly.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Continuously tunable multistability in DNA replication networks.Nature communications · 2026Article
- Synthetic dynamic transcription frameworks and their applications.Nature chemistry · 2026Review
- Dynamic Control of DNA Origami Self-Assembly by Transcriptional Modules.Journal of the American Chemical Society · 2026Article
- A Modular T7 RNA Polymerase Toolbox Linking Selective miRNA Detection to Signal Amplification and Protein Expression.Journal of the American Chemical Society · 2025Article
- Diversifying Substrates and Reaction Conditions for Polymerase Strand Recycling.ACS synthetic biology · 2025Article
- Adenosine-Triggered Dynamic and Transient Aptamer-Based Networks Integrated in Liposome Protocell Assemblies.Journal of the American Chemical Society · 2025Article
- Dynamic Transcription Machineries in Protocells.Journal of the American Chemical Society · 2025Review
- A cell-free biosensor signal amplification circuit with polymerase strand recycling.Nature chemical biology · 2025Article
- Temporal Contrastive Learning through implicit non-equilibrium memory.Nature communications · 2025Article
- Covalent Dynamic DNA Networks to Translate Multiple Inputs into Programmable Outputs.Journal of the American Chemical Society · 2025Article
- Bistable Functions and Signaling Motifs in Systems Chemistry: Taking the Next Step Toward Synthetic Cells.Accounts of chemical research · 2025Review
- Photochemically Triggered, Transient, and Oscillatory Transcription Machineries Guide Temporal Modulation of Fibrinogenesis.Journal of the American Chemical Society · 2025Article
- Spatial Control over Reactions via Localized Transcription within Membraneless DNA Nanostar Droplets.Journal of the American Chemical Society · 2024Article
- Directing Nanoparticle Organization in Response to Diverse Chemical Inputs.Journal of the American Chemical Society · 2024Article
- Developmental assembly of multi-component polymer systems through interconnected synthetic gene networks in vitro.Nature communications · 2024Article
- Strategies to Reduce Promoter-Independent Transcription of DNA Nanostructures and Strand Displacement Complexes.ACS synthetic biology · 2024Review
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems.Nature communications · 2024Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of cellular dynamics and responses to stimuli by genetic regulatory networks suggests how in vitro chemical reaction networks might analogously direct the dynamics of synthetic materials or chemistries. A key step in developing genetic regulatory network analogues capable of this type of sophisticated regulation is the integration of multiple coordinated functions within a single network. Here, we demonstrate how such functional integration can be achieved using in vitro transcriptional genelet circuits that emulate essential features of cellular genetic regulatory networks. By successively incorporating functional genelet modules into a bistable circuit, we construct an integrated regulatory network that dynamically changes its state in response to upstream stimuli and coordinates the timing of downstream signal expression. We use quantitative models to guide module integration and develop strategies to mitigate undesired interactions between network components that arise as the size of the network increases. This approach could enable the construction of in vitro networks capable of multifaceted chemical and material regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.