Evidence map›Paper›PMID 31427757›Full record

ArticleNature methods2019

Oblique-plane single-molecule localization microscopy for tissues and small intact animals.

Jeongmin Kim, Michal Wojcik, Yuan Wang, Seonah Moon, Emilia A Zin, Nadia Marnani, Zachary L Newman, John G Flannery, Ke Xu, Xiang Zhang

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Nature methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
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  9. MINFLUX fluorescence nanoscopy in biological tissue.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  10. Article
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  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023
    Article
  18. Article
  19. Review
  20. Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Jeongmin KimNSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-1235-1487
Michal WojcikDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-0053-1018
Yuan WangNSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA.
Seonah MoonDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
Emilia A ZinVision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA.
Nadia MarnaniDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Zachary L NewmanDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
John G FlanneryVision Science, School of Optometry, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-0720-8897
Ke XuDepartment of Chemistry, University of California, Berkeley, Berkeley, CA, USA. xuk@berkeley.edu.ORCID http://orcid.org/0000-0002-2788-194X
Xiang ZhangNSF Nanoscale Science and Engineering Center, University of California, Berkeley, Berkeley, CA, USA. xiang@berkeley.edu.ORCID http://orcid.org/0000-0002-3272-894X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 µm for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.

Indexed as

A549 CellsAnimalsBrainCaenorhabditis elegansDrosophila melanogasterFemaleFishesHumansImaging, Three-DimensionalMaleMiceMice, Inbred C57BLMicroscopy, FluorescenceRetinaSingle Molecule Imaging

Identifiers

PMID31427757

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.