ArticleThe EMBO journal1988
Porcine vinculin and metavinculin differ by a 68-residue insert located close to the carboxy-terminal part of the molecule.
Article in The EMBO journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- The role of extracellular matrix stiffness in regulating cytoskeletal remodeling via vinculin in synthetic smooth muscle cells.Biochemical and biophysical research communications · 2019Article
- Conformational states during vinculin unlocking differentially regulate focal adhesion properties.Scientific reports · 2018Article
- Vinculin in cell-cell and cell-matrix adhesions.Cellular and molecular life sciences : CMLS · 2017Review
- Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments.Journal of molecular biology · 2015Article
- The metavinculin tail domain directs constitutive interactions with raver1 and vinculin RNA.Journal of molecular biology · 2012Article
- The C-terminal tail domain of metavinculin, vinculin's splice variant, severs actin filaments.The Journal of cell biology · 2012Article
- Length-dependent modulation of cytoskeletal remodeling and mechanical energetics in airway smooth muscle.American journal of respiratory cell and molecular biology · 2011Article
- Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein.The Journal of biological chemistry · 2010Article
- Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin.The Journal of cell biology · 1994Article
- Complementary distributions of vinculin and dystrophin define two distinct sarcolemma domains in smooth muscle.The Journal of cell biology · 1993Article
- Dystrophin colocalizes with beta-spectrin in distinct subsarcolemmal domains in mammalian skeletal muscle.The Journal of cell biology · 1992Article
- Complete sequence of human vinculin and assignment of the gene to chromosome 10.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Antibody mapping of functional domains in vinculin.The EMBO journal · 1990Article
- Identification of two distinct functional domains on vinculin involved in its association with focal contacts.The Journal of cell biology · 1989Article
- Primary sequence and domain structure of chicken vinculin.The Biochemical journal · 1989Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metavinculin is a higher mol. wt variant of vinculin expressed only in muscle tissue. Using amino acid sequencing methods on the intact molecules and their proteolytic subfragments, together with a polyclonal antibody specific only for metavinculin from porcine stomach, we have been able to identify and sequence the difference peptide in the porcine metavinculin molecule. By alignment with the complete sequence of chick fibroblast vinculin (communicated by G.J. Price, P. Jones, M.D. Davison, R. Bendori, S. Griffiths, B. Patel, B. Geiger and D.R. Critchley, prior to publication) the exact location of the insert could be determined. In porcine metavinculin, this insert lies between the 90-kd protease-resistant amino-terminal core and the carboxy terminus of the molecule. It contains 68 amino acids and is flanked by KWSSK sequences, one of which is present in vinculin. The identity of the mapped vinculin and metavinculin sequences outside this difference peptide is consistent with the two proteins arising via alternative splicing at the mRNA level. The lack of reactivity of the porcine metavinculin antibody with metavinculin from chicken as well as the finding of different proteolytic cleavage sites in avian metavinculin indicate a species-specific amino acid sequence in the difference piece of the metavinculin molecule.
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