ArticleScientific reports2019
A flow through device for simultaneous dielectrophoretic cell trapping and AC electroporation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
- Structural, Compositional, and Dielectric State Profiling in Label-Free Single-Cell Monitoring.Small methods · 2026Review
- A critical review of microfluidic electroporation for therapeutic cell engineering.Cell reports. Physical science · 2026Article
- Strategies to control cellular spatial organization in microphysiological systems.Microsystems & nanoengineering · 2026Review
- Analysis of Cell Dielectrophoretic Properties Using Isomotive Creek-Gap Electrode Device.Sensors (Basel, Switzerland) · 2024Article
- Recent Advances in Dielectrophoretic Manipulation and Separation of Microparticles and Biological Cells.Biosensors · 2024Review
- On-chip dielectrophoretic single-cell manipulation.Microsystems & nanoengineering · 2024Review
- Engineered 3D liver-tissue model with minispheroids formed by a bioprinting process supported withBioactive materials · 2024Article
- Single-Cell RNA Sequencing in Organ and Cell Transplantation.Biosensors · 2024Review
- Portable dielectrophoresis for biology: ADEPT facilitates cell trapping, separation, and interactions.Microsystems & nanoengineering · 2024Article
- Magnetophoretic circuits: A review of device designs and implementation for precise single-cell manipulation.Analytica chimica acta · 2023Review
- Highly efficient cell-microbead encapsulation using dielectrophoresis-assisted dual-nanowell array.PNAS nexus · 2023Article
- New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation.Biosensors · 2023Article
- Protein Dielectrophoresis with Gradient Array of Conductive Electrodes Sheds New Light on Empirical Theory.Analytical chemistry · 2023Article
- Microfluidic Systems for Blood and Blood Cell Characterization.Biosensors · 2022Review
- Electro-Microfluidic Assembly Platform for Manipulating Colloidal Structures inside Water-in-Oil Emulsion Droplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Magnetophoretic capacitors for storing single particles and magnetized cells in microfluidic devices.Biomicrofluidics · 2022Article
- Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system.Engineering in life sciences · 2022Article
- Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition.Frontiers in bioengineering and biotechnology · 2022Article
- Numerical Study of Joule Heating Effects on Microfluidics Device Reliability in Electrode Based Devices.Materials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of cells and their transfection in a controlled manner is an integral step in cell biotechnology. Electric field approaches such as dielectrophoresis (DEP) offers a more viable method for targeted immobilization of cells without any labels. For transfection of cells to incorporate exogenous materials, electrical methods such as electroporation, are preferred over chemical and viral delivery methods since they minimally affect cell viability and can target many types. However prior approaches to both methods required multiple excitation sources, an AC source for DEP-based trapping and another DC source for electroporation. In this paper, we present a first of its kind flow through lab-on-chip platform using a single AC excitation source for combined trapping using negative dielectrophoresis (nDEP) and AC electroporation. Use of AC fields for electroporation eliminates the unwanted side effects of electrolysis or joule heating at electrodes compared to DC electroporation. Adjusting the flow rate and the electrical parameters of the incident AC field precisely controls the operation (trap, trap with electroporation and release). The platform has been validated through trapping and simultaneous transfection of HEK-293 embryonic kidney cells with a plasmid vector containing a fluorescent protein tag. Numerical scaling analysis is provided that indicates promise for individual cell trapping and electroporation using low voltage AC fields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.