ArticleScientific reports2019
De-epithelialization of porcine tracheal allografts as an approach for tracheal tissue engineering.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Tracheal Regeneration: Recent Progress in the Application of Stem Cells in Tracheal Bioengineering.International journal of molecular sciences · 2026Review
- Ex vivo perfusion recellularization of acellular rat hindlimbs for vascular composite allotransplantation.Scientific reports · 2025Article
- In situ vascularization and epithelialization of segmental bioengineered trachea based on marrow-derived stem/progenitor cells.Materials today. Bio · 2025Article
- Assessing the Impact of Partial Decellularization on Tracheal Chondrocytes and Extracellular Matrix in Airway Reconstruction.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2025Article
- Development and Characterization of a Primary Ciliated Porcine Airway Model for the Evaluation of In Vitro Mucociliary Clearance and Mucosal Drug Delivery.Pharmaceutics · 2025Article
- Recent Advances in the Application of 3D-Printing Bioinks Based on Decellularized Extracellular Matrix in Tissue Engineering.ACS omega · 2024Review
- Dynamic flow for efficient partial decellularization of tracheal grafts: A preliminary rabbit study.Laryngoscope investigative otolaryngology · 2024Article
- Long-Term Chondrocyte Retention in Partially Decellularized Tracheal Grafts.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2024Article
- In silico model development and optimization of in vitro lung cell population growth.PloS one · 2024Article
- Evaluation of a decellularized bronchial patch transplant in a porcine model.Scientific reports · 2023Article
- Partial decellularization eliminates immunogenicity in tracheal allografts.Bioengineering & translational medicine · 2023Article
- Regeneration of tracheal neotissue in partially decellularized scaffolds.NPJ Regenerative medicine · 2023Article
- Biomaterial-based scaffolds in promotion of cartilage regeneration: Recent advances and emerging applications.Journal of orthopaedic translation · 2023Review
- Orthotopic Ferret Tracheal Transplantation Using a Recellularized Bioengineered Graft Produces Functional Epithelia.Bioengineering (Basel, Switzerland) · 2023Article
- Investigation of the In Vitro and In Vivo Biocompatibility of a Three-Dimensional Printed Thermoplastic Polyurethane/Polylactic Acid Blend for the Development of Tracheal Scaffolds.Bioengineering (Basel, Switzerland) · 2023Article
- Article
- A Multimodal Approach to Quantify Chondrocyte Viability for Airway Tissue Engineering.The Laryngoscope · 2023Article
- Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- Recellularization of Bioengineered Scaffolds for Vascular Composite Allotransplantation.Frontiers in surgery · 2022Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replacement of large tracheal defects remains an unmet clinical need. While recellularization of acellular tracheal grafts appeared to be a viable pathway, evidence from the clinic suggests otherwise. In hindsight, complete removal of chondrocytes and repopulation of the tracheal chondroid matrix to achieve functional tracheal cartilage may have been unrealistic. In contrast, the concept of a hybrid graft whereby the epithelium is removed and the immune-privileged cartilage is preserved is a radically different path with initial reports indicating potential clinical success. Here, we present a novel approach using a double-chamber bioreactor to de-epithelialize tracheal grafts and subsequently repopulate the grafts with exogenous cells. A 3 h treatment with sodium dodecyl sulfate perfused through the inner chamber efficiently removes the majority of the tracheal epithelium while the outer chamber, perfused with growth media, keeps most (68.6 ± 7.3%) of the chondrocyte population viable. De-epithelialized grafts support human bronchial epithelial cell (BEAS-2B) attachment, viability and growth over 7 days. While not without limitations, our approach suggests value in the ultimate use of a chimeric allograft with intact donor cartilage re-epithelialized with recipient-derived epithelium. By adopting a brief and partial decellularization approach, specifically removing the epithelium, we avoid the need for cartilage regeneration.
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