Evidence map›Paper›PMID 31427528›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2019

Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes.

Chungyu Chang, Chenggang Wu, Jerzy Osipiuk, Sara D Siegel, Shiwei Zhu, Xiangan Liu, Andrzej Joachimiak, Robert T Clubb, Asis Das, Hung Ton-That

Open access · bronzeAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  6. Review
  7. Article
  8. Article
  9. LrpCBA pilus proteins of gut-dwelling Ligilactobacillus ruminis: crystallization and X-ray diffraction analysis.Acta crystallographica. Section F, Structural biology communications · 2021
    Article
  10. Review
  11. Frontiers in microbiology · 2021
    Review
  12. Review
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 1 country.

Chungyu ChangDivision of Oral Biology and Medicine, School of Dentistry, University of California, Los Angeles, CA 90095; jchang@dentistry.ucla.edu htonthat@dentistry.ucla.edu.
Chenggang WuDepartment of Microbiology & Molecular Genetics, University of Texas Health Science Center, Houston, TX 77030.
Jerzy OsipiukCenter for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60637.
Sara D SiegelDepartment of Microbiology & Molecular Genetics, University of Texas Health Science Center, Houston, TX 77030.
Shiwei ZhuDepartment of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT 06510.
Xiangan LiuDepartment of Microbiology & Molecular Genetics, University of Texas Health Science Center, Houston, TX 77030.
Andrzej JoachimiakCenter for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60637.
Robert T ClubbDepartment of Chemistry and Biochemistry, University of California, Los Angeles-Department of Energy Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095.
Asis DasDepartment of Medicine, Neag Comprehensive Cancer Center, University of Connecticut Health Center, Farmington, CT 06030.
Hung Ton-ThatDivision of Oral Biology and Medicine, School of Dentistry, University of California, Los Angeles, CA 90095; jchang@dentistry.ucla.edu htonthat@dentistry.ucla.edu.
The University of Texas Health Science Center at Houston · USUniversity of California, Los Angeles · USJoint Center for Structural Genomics · USUConn Health · USUniversity of Chicago · USYale University · US

Funding

Cell Surface Protein Anchoring in Gram-Positive BacteriaR01AI052217 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Thompson Clubb · 2002 to 2026
$8.5M
Molecular Assembly on the Cell Surface of ActinomycesR01DE017382 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI Hung Ton-That · 2008 to 2026
$6.7M
Molecular mechanisms of polymicrobial interactions in Fusobacterium nucleatumR21DE026574 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI WU, CHENGGANG · 2017 to 2018
$424k
Mechanism of pilus hijacking and adherence by the coaggregation factor CafA in Actinomyces orisF31DE027295 · NIDCR · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SIEGEL, SARA DANIELLE · 2017 to 2018
$41k
NIAID NIH HHS HHSN272201200026CNIAID NIH HHS HHSN272201700060CNIAID NIH HHS R01 AI052217NIDCR NIH HHS F31 DE027295NIDCR NIH HHS R01 DE017382NIDCR NIH HHS R21 DE026574
6 · The paper itself

Abstract

Assembly of pili on the gram-positive bacterial cell wall involves 2 conserved transpeptidase enzymes named sortases: One for polymerization of pilin subunits and another for anchoring pili to peptidoglycan. How this machine controls pilus length and whether pilus length is critical for cell-to-cell interactions remain unknown. We report here in

Indexed as

ActinomycesAdhesins, BacterialAminoacyltransferasesBacterial ProteinsCysteine EndopeptidasesFimbriae, BacterialAdhesins, BacterialAminoacyltransferasesBacterial ProteinsCysteine Endopeptidasessortase Asortase Ccoaggregationgram-positive bacteriapilus assemblypilus lengthsortase

Identifiers

PMID31427528
PMCPMC6731673
OpenAlexW2969236460

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.