ArticleBMC complementary and alternative medicine2019
Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells.
Article in BMC complementary and alternative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
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- Chemo-Diversity and Secondary Metabolite Content in Corticolous Crustose Lichens (Fam. Arthoniaceae, Graphidaceae, Pyrenulaceae) from Remnants of Colombian Tropical Dry Forests.Journal of fungi (Basel, Switzerland) · 2026Article
- Phytochemical Profiling and Antioxidant and Enzymatic Evaluation of Extracts from the Antarctic LichensMolecules (Basel, Switzerland) · 2026Article
- Yttrium oxide nanoparticles induce potent selective cytotoxicity in HeLa cervical cance cells through ROS-mediated genomic instability and mitochondrial apoptosis.Scientific reports · 2026Article
- The Identification of Gyrophoric Acid, a Phytochemical Derived from Lichen, as a Potent Inhibitor for Aggregation of Amyloid Beta Peptide: In Silico and Biochemical Evaluation.International journal of molecular sciences · 2025Article
- A Comprehensive Review on Chemical Structures and Bioactivities ofJournal of fungi (Basel, Switzerland) · 2025Review
- Antiproliferative and pro-apoptotic effects ofFrontiers in oncology · 2025Article
- Gyrophoric Acid, a Secondary Metabolite of Lichens, Exhibits Antidepressant and Anxiolytic Activity In Vivo in Wistar Rats.International journal of molecular sciences · 2024Article
- Review
- Screening Evaluation of Antiproliferative, Antimicrobial and Antioxidant Activity of Lichen Extracts and Secondary Metabolites In Vitro.Plants (Basel, Switzerland) · 2023Article
- Lichen Depsides and Tridepsides: Progress in Pharmacological Approaches.Journal of fungi (Basel, Switzerland) · 2023Review
- Tridepsides as potential bioactives: a review on their chemistry and the global distribution of their lichenic and non-lichenic natural sources.Frontiers in fungal biology · 2023Review
- A Comparative Study of Isolated Secondary Metabolites from Lichens and Their Antioxidative Properties.Plants (Basel, Switzerland) · 2022Article
- Biological Effects of Gyrophoric Acid and Other Lichen Derived Metabolites, on Cell Proliferation, Apoptosis and Cell Signaling pathways.Chemico-biological interactions · 2022Review
- Pro-Apoptotic Potential ofPharmaceutics · 2021Article
- Tropical lichen, Dirinaria consimilis, induces ROS-mediated activation of MAPKs and triggers caspase cascade mediated apoptosis in brain and cervical cancer cells.Molecular and cellular biochemistry · 2021Article
- Potential Effect ofBiomolecules · 2021Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundLichens produce a huge diversity of bioactive compounds with several biological effects. Gyrophoric acid (GA) is found in high concentrations in the common lichen Umbilicaria hirsuta, however evidence for biological activity was limited to anti-proliferative activity described on several cancer cell lines.
methodsWe developed and validated a new protocol for GA isolation, resulting in a high yield of highly pure GA (validated by HPLC and NMR) in an easy and time saving manner. Anti-proliferative and pro-apoptotic activity, oxygen radicals formation and stress/survival proteins activity changes was study by flow cytometry.
resultsThe highly purified GA showed anti-proliferative activity against HeLa (human cervix carcinoma) and other tumor cells. Moreover, GA threated cells showed a significant increase in caspase-3 activation followed by PARP cleavage, PS externalization and cell cycle changes mediated by oxidative stress. Production of oxygen radicals led to DNA damage and changes in stress/survival pathways activation.
conclusionsGA treatment on HeLa cells clearly indicates ROS production and apoptosis as form of occurred cell death. Moreover, DNA damage and changing activity of stress/survival proteins as p38MAPK, Erk1/2 and Akt mediated by GA treatment confirm pro-apoptotic potential. The pharmacological potential of U. hirsuta derived GA is discussed.
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