Evidence map›Paper›PMID 31426793›Full record

ArticleBMC veterinary research2019

Establishment and evaluation of a PRRSV-sensitive porcine endometrial epithelial cell line by transfecting SV40 large T antigen.

Kang Zhang, Houshen Li, Shasha Dong, Ying Liu, Dong Wang, Haichang Liu, Feng Su, Lijiang Ge, Yunliang Jiang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Roles of MOV10 in Animal RNA Virus Infection.Frontiers in veterinary science · 2020
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kang ZhangShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China.
Houshen LiCollege of Chemistry and Material Science, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China.
Shasha DongDepartment of Cardiology, Shandong First Medical University and Shandong Academy of Medical Science, Taian, 271000, China.
Ying LiuShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China.
Dong WangShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China.
Haichang LiuNingbo Defangyuan Biotech Co. Ltd., No.166 of Xinghaibei Road, County of Ninghai, Ningbo, Zhejiang, China.
Feng SuShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China. suf@sdau.edu.cn.ORCID http://orcid.org/0000-0001-8722-9606
Lijiang GeShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China. glj@sdau.edu.cn.
Yunliang JiangShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 271018, No. 61 Daizong Street, Taian, Shandong, China. yljiang723@aliyun.com.

Funding

Agricultural Breed Project of Shandong Province 2016Shandong Double Tops Program No. SYL2017YSTD12
6 · The paper itself

Abstract

backgroundPRRSV is an infectious illness causing lung injury and abortion in sows. Cells apoptosis in the interface between the endometrium and fetal placenta is a crucial factor causing abortion. Previous study confirmed PRRSV could cause apoptosis of macrophages but rarely produced an obvious change in porcine endometrial epithelial cells (PECs). Recently, PRRSV-induced abortion was attributed to fetal placental and endometrium epithelial cells (Sn

resultsIn this study, highly purified primary PECs were harvested through differential digestion, and their characteristics were confirmed by CK18, ERɑ and PR staining. Cells were then immortalized by transfecting a lentiviral vector that expressed SV40 large T antigen. PECs lines were obtained after puromycin screening. Proliferation of cell line was evaluated by cell growth curve and cell cycle assays. Cell lines exhibited faster proliferation capacity than primary cells. Biological characteristics of cell line were assessed by Western blot, karyotype analysis and staining, which confirmed that the cell line retained the endometrium characteristics. Finally, PRRSV sensitivity was assessed; expression of Sn and CD163 indicated that primary PECs and cell lines were all potentially sensitive to PRRSV. PRRSV infection tests showed an obvious increase in apoptotic rate in the infected PEC cell line, which suggested its susceptibility.

conclusionThe newly constructed cell line is a useful tool for studying the mechanism of abortion caused by PRRSV.

Indexed as

AnimalsAntigens, Polyomavirus TransformingCell Culture TechniquesCell LineEpithelial CellsPorcine respiratory and reproductive syndrome virusSwineVirus CultivationAntigens, Polyomavirus TransformingLentrival vectorPorcine endometrial epithelial cell linePRRSV-sensitivityReplication efficiencySV40T antigen

Identifiers

PMID31426793
PMCPMC6700808

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.