ArticleBMC plant biology2019
Integrative omics analysis on phytohormones involved in oil palm seed germination.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Combined transcriptomic and metabolomic analysis of the molecular mechanism of seed dormancy release in Notopterygium incisum.BMC genomics · 2025Article
- Comparative Transcriptome and Hormonal Analysis Reveals the Mechanisms of Salt Tolerance in Rice.International journal of molecular sciences · 2025Article
- GWAS determined genetic loci associated with callus induction in oil palm tissue culture.Plant cell reports · 2024Article
- Genome-Wide Identification of Homeodomain Leucine Zipper (HD-ZIP) Transcription Factor, Expression Analysis, and Protein Interaction of HD-ZIP IV in Oil Palm Somatic Embryogenesis.International journal of molecular sciences · 2023Article
- Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress.International journal of molecular sciences · 2022Article
- Underlying Biochemical and Molecular Mechanisms for Seed Germination.International journal of molecular sciences · 2022Review
- Transcriptome analysis reveals key developmental and metabolic regulatory aspects of oil palm (Elaeis guineensis Jacq.) during zygotic embryo development.BMC plant biology · 2022Article
- Beta-Amylase and Phosphatidic Acid Involved in Recalcitrant Seed Germination of Chinese Chestnut.Frontiers in plant science · 2022Article
- Roles of Reactive Oxygen Species and Mitochondria in Seed Germination.Frontiers in plant science · 2021Review
- Seed Germination in Oil Palm (International journal of molecular sciences · 2020Review
- Overexpression of MaizeFrontiers in plant science · 2020Article
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundHeat treatment is widely used to break dormancy for seed germination and phytohormones could be deeply involved. However, effect of heat treatment on phytohormone related genes/proteins/metabolites and possible relationship with dormancy release remains unclear in oil palm. In this study, oil palm seeds were heat-treated at 39 °C for 60 days according to the method for commercial production. The embryos of seeds during heat treatment (0 d, 15 d, 30 d, 45 d and 60 d) and of germinated seeds (70 d and 75 d) were selected to discover the mechanisms involved in oil palm seed germination. RNA-seq and iTRAQ were applied to investigate DEGs and DEPs related to seed germination; qPCR and western blot were used as validation accordingly; endogenous phytohormones were determined by LC-MS/MS and exogenous phytohormones were also applied to validate their effects on seed germination.
resultsRNA-seq results showed that plant hormone signal transduction was one of the most important pathways and eight phytohormones involved, while six of them (ABA, GA, ET, CTK, IAA and JA) were also identified by iTRAQ. Both RNA-seq and iTRAQ results showed that the expression of ABA decreased after heat treatment, which was further validated by qPCR and western blot. Furthermore, changes in endogenous phytohormones showed that ABA decreased rapidly to about 9% of the control at 30 d and then stayed at very low levels until germination; GA and CTK increased while IAA was not affected by heat treatment. Besides, exogenous ABA treatments (10, 100, 1000 mg/L) showed that the germination rate decreased to 63, 42 and 16% of the control, respectively, suggesting that ABA suppress seed germination and the inhibition effect increase with higher concentration; while the germination rates of exogenous GA and IAA treatments barely changed among different concentrations.
conclusionsPhytohormones are deeply involved in oil palm seed germination and ABA acts as an inhibitor. Heat treatment can eliminate endogenous ABA and break dormancy, while GA and CTK may also involve in dormancy release. At least 30 days of heat treatment might be necessary. This study provided informative perspectives on oil palm seed germination, which could be also applicable in other palm species.
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