ArticleInternational journal of molecular sciences2019
A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Zinc-mediated lysosomal activation by 1H10 enhances autophagy and attenuates tau pathology in Alzheimer's disease models.Molecular brain · 2026Article
- ZnbioRxiv : the preprint server for biology · 2026Article
- Trace element-linked DNA methylation sites and their association with type 2 diabetes and cardiovascular diseases: EPIC-Potsdam cohort study.Clinical epigenetics · 2025Article
- Trace elements and risk of diabetes-related vascular complications: results from the EPIC-Potsdam cohort study.Cardiovascular diabetology · 2025Article
- Zinc eluted from glassware is a risk factor for embryo development in human and animal assisted reproduction†.Biology of reproduction · 2025Article
- Human genetic variants in SLC39A8 impact uptake and steady-state metal levels within the cell.Life science alliance · 2025Article
- Total and Free Zinc Dynamics as Biomarkers for Neurological Impairment in Traumatic Spinal Cord Injury.Nutrients · 2025Article
- Translational Algorithms for Technological Dietary Quality Assessment Integrating Nutrimetabolic Data with Machine Learning Methods.Nutrients · 2024Article
- Postprandial Micronutrient Variability and Bioavailability: An Interventional Meal Study in Young vs. Old Participants.Nutrients · 2024Article
- Discoveries of GPR39 as an evolutionarily conserved receptor for bile acids and of its involvement in biliary acute pancreatitis.Science advances · 2024Article
- Selenium, Zinc, and Copper Status of Vegetarians and Vegans in Comparison to Omnivores in the Nutritional Evaluation (NuEva) Study.Nutrients · 2023Article
- A fluorometric assay to determine labile copper(II) ions in serum.Scientific reports · 2023Article
- Excessive copper impairs intrahepatocyte trafficking and secretion of selenoprotein P.Nature communications · 2023Article
- TC-G 1008 facilitates epileptogenesis by acting selectively at the GPR39 receptor but non-selectively activates CREB in the hippocampus of pentylenetetrazole-kindled mice.Cellular and molecular life sciences : CMLS · 2023Article
- Nutritional deficiencies that may predispose to long COVID.Inflammopharmacology · 2023Review
- First review on the selenium status in Germany covering the last 50 years and on the selenium content of selected food items.European journal of nutrition · 2023Review
- Albumin-mediated extracellular zinc speciation drives cellular zinc uptake.Chemical communications (Cambridge, England) · 2022Article
- Article
- Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free ZnACS chemical biology · 2022Article
- Free Zinc as a Predictive Marker for COVID-19 Mortality Risk.Nutrients · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Zinc is an essential trace element, making it crucial to have a reliable biomarker for evaluating an individual's zinc status. The total serum zinc concentration, which is presently the most commonly used biomarker, is not ideal for this purpose, but a superior alternative is still missing. The free zinc concentration, which describes the fraction of zinc that is only loosely bound and easily exchangeable, has been proposed for this purpose, as it reflects the highly bioavailable part of serum zinc. This report presents a fluorescence-based method for determining the free zinc concentration in human serum samples, using the fluorescent probe Zinpyr-1. The assay has been applied on 154 commercially obtained human serum samples. Measured free zinc concentrations ranged from 0.09 to 0.42 nM with a mean of 0.22 ± 0.05 nM. It did not correlate with age or the total serum concentrations of zinc, manganese, iron or selenium. A negative correlation between the concentration of free zinc and total copper has been seen for sera from females. In addition, the free zinc concentration in sera from females (0.21 ± 0.05 nM) was significantly lower than in males (0.23 ± 0.06 nM). The assay uses a sample volume of less than 10 µL, is rapid and cost-effective and allows us to address questions regarding factors influencing the free serum zinc concentration, its connection with the body's zinc status, and its suitability as a future biomarker for an individual's zinc status.
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Registered trials
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