Evidence map›Paper›PMID 31426445›Full record

ArticleCancers2019

Single-Cell Analysis of Multiple Steps of Dynamic NF-κB Regulation in Interleukin-1α-Triggered Tumor Cells Using Proximity Ligation Assays.

Christin Mayr-Buro, Eva Schlereth, Knut Beuerlein, Ulas Tenekeci, Johanna Meier-Soelch, M Lienhard Schmitz, Michael Kracht

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Christin Mayr-BuroRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany. christin.mayr-buro@pharma.med.uni-giessen.de.
Eva SchlerethRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany.
Knut BeuerleinRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany.
Ulas TenekeciRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany.
Johanna Meier-SoelchRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany.
M Lienhard SchmitzInstitute of Biochemistry, Justus Liebig University, D-35392 Giessen, Germany. lienhard.schmitz@biochemie.med.uni-giessen.de.
Michael KrachtRudolf-Buchheim-Institute of Pharmacology, Justus Liebig University, D-35392 Giessen, Germany. michael.kracht@pharma.med.uni-giessen.de.
Justus-Liebig-Universität Gießen · DE

Funding

Deutsche Forschungsgemeinschaft 109546710Deutsche Forschungsgemeinschaft 197785619Deutsche Forschungsgemeinschaft Cardio Pulmonary Institute (CPI)Deutsche Forschungsgemeinschaft ECCPS, EXC 147/2Deutsche Forschungsgemeinschaft KR1141/9-1 (KLIFO309)
6 · The paper itself

Abstract

The frequently occurring heterogeneity of cancer cells and their functional interaction with immune cells in the tumor microenvironment raises the need to study signaling pathways at the single cell level with high precision, sensitivity, and spatial resolution. As aberrant NF-κB activity has been implicated in almost all steps of cancer development, we analyzed the dynamic regulation and activation status of the canonical NF-κB pathway in control and IL-1α-stimulated individual cells using proximity ligation assays (PLAs). These systematic experiments allowed the visualization of the dynamic dissociation and re-formation of endogenous p65/IκBα complexes and the nuclear translocation of NF-κB p50/p65 dimers. PLA combined with immunostaining for p65 or with

Indexed as

DCP1ainterleukin-1 signalingNF-κBP-bodiesposttranscriptional gene regulationproximity ligation assayssingle cell analysistranscriptiontumor heterogeneity

Identifiers

PMID31426445
PMCPMC6721548
OpenAlexW2968014495

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.