ArticleStructure (London, England : 1993)2019
Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.
Article in Structure (London, England : 1993), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Ligand Screening and Discovery Using Cocktail Soaking and Automated Microcrystal Electron Diffraction.ChemMedChem · 2025Article
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Structure of the lens MP20 mediated adhesive junction.Nature communications · 2025Article
- Unraveling atomic complexity from frozen samples.Structural dynamics (Melville, N.Y.) · 2025Article
- Ligand Screening and Discovery using Cocktail Soaking and Automated MicroED.bioRxiv : the preprint server for biology · 2025Article
- In situ crystalline structure of the human eosinophil major basic protein-1.bioRxiv : the preprint server for biology · 2024Article
- Eliminating the missing cone challenge through innovative approaches.Journal of structural biology: X · 2024Article
- Structure of the lens MP20 mediated adhesive junction.bioRxiv : the preprint server for biology · 2024Article
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- Article
- A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.Nature communications · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Review
- Benchmarking the ideal sample thickness in cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).STAR protocols · 2021Article
- MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A simple pressure-assisted method for MicroED specimen preparation.Nature communications · 2021Article
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.
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Registered trials
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