ArticleReproductive biology and endocrinology : RB&E2019
MicroRNA-200b and microRNA-200c are up-regulated in PCOS granulosa cell and inhibit KGN cell proliferation via targeting PTEN.
Article in Reproductive biology and endocrinology : RB&E, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- ANGPTL4 induces granulosa cells proliferation dysfunction in PCOS by regulating the immune microenvironment and WNT signaling pathway.Journal of ovarian research · 2026Article
- MicroRNA Crossroads in PCOS and Endometriosis.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Screening and analysis of plasma small extracellular vesicle MiRNAs at various estrous cycle phases of goat.Journal of ovarian research · 2025Article
- Mechanistic Study of MiR-30c-5p Regulation of SIRT Expression in Polycystic Ovary Syndrome.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Non-coding molecular determinants of polycystic ovary syndrome: A future treatment potential.Journal of assisted reproduction and genetics · 2025Review
- Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis.Journal of extracellular biology · 2025Article
- Granulosa Cells-Related MicroRNAs in Ovarian Diseases: Mechanism, Facts and Perspectives.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
- Knockdown of miR-24 Suppressed the Tumor Growth of Cervical Carcinoma Through Regulating PTEN/PI3K/AKT Signaling Pathway.Biochemical genetics · 2024Article
- Linoleic acid induces human ovarian granulosa cell inflammation and apoptosis through the ER-FOXO1-ROS-NFκB pathway.Scientific reports · 2024Article
- Function of MicroRNAs in Normal and Abnormal Ovarian Activities: A Review Focus on MicroRNA-21.International journal of fertility & sterility · 2024Article
- A rat study on the PTEN expression in ovarian tissue in PCOS and folliculogenesis.Scientific reports · 2023Article
- Integrating network pharmacology and experimental verification to explore the pharmacological mechanisms of asparagus against polycystic ovary syndrome.Journal of ovarian research · 2023Article
- miR-4433a-3p promotes granulosa cell apoptosis by targeting peroxisome proliferator-activated receptor alpha and inducing immune cell infiltration in polycystic ovarian syndrome.Journal of assisted reproduction and genetics · 2023Article
- Multi-omics insights and therapeutic implications in polycystic ovary syndrome: a review.Functional & integrative genomics · 2023Review
- The physiological functions and therapeutic potential of exosomes during the development and treatment of polycystic ovary syndrome.Frontiers in physiology · 2023Review
- Differences in metabonomic profiles of abdominal subcutaneous adipose tissue in women with polycystic ovary syndrome.Frontiers in endocrinology · 2023Article
- Overexpression of miR-144-3p alleviates polycystic ovaries syndrome through targeting expression of HSP-70.Gene therapy · 2022Article
- Follicular Fluid-Derived Exosomal MicroRNA-18b-5p Regulates PTEN-Mediated PI3K/Akt/mTOR Signaling Pathway to Inhibit Polycystic Ovary Syndrome Development.Molecular neurobiology · 2022Article
- Knockdown of DPP4 promotes the proliferation and the activation of the CREB/aromatase pathway in ovarian granulosa cells.Molecular medicine reports · 2022Article
- Comprehensive Assessment of the STIMs and Orais Expression in Polycystic Ovary Syndrome.Frontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction. Several studies have reported that the aberrant expression of miRNAs contributes a lot to disordered folliculogenesis in PCOS, though the role and underlying mechanism of microRNA-200b (miR-200b) and microRNA-200c (miR-200c) in the development of PCOS remain unclear.
methodsThe expression of miR-200b in granulosa cells (GCs) derived from 90 PCOS patients and 70 controls was analyzed by using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Granulosa-like tumor cell line (KGN) was cultured for cell counting kit-8 (CCK-8) assays after over-expression of miR-200b, miR-200c or knockdown phosphatase and tensin homolog (PTEN). TargetScan was used to identify the potential targets of miR-200b and miR-200c, which was further verified by qRT-PCR, western blot and luciferase assays.
resultsSignificantly increased expression of miR-200b was observed in PCOS patients compared with the controls. Moreover, over-expression of miR-200b and miR-200c inhibited the proliferation of KGN cells. In addition, our results verified that miR-200b and miR-200c directly targeted PTEN, knockdown of which suppressed KGN cells proliferation.
conclusionOur findings demonstrate that miR-200b and miR-200c suppress the proliferation of KGN cells by targeting PTEN, and this might provide new evidence for abnormal proliferation of GCs in PCOS.
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