Evidence map›Paper›PMID 31421107›Full record

ArticleThe Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia.

Desak Nyoman Surya Suameitria Dewi, Ni Made Mertaniasih, Soedarsono, Yuriko Ozeki, Wayan Tunas Artama, Fihiruddin, Mamiko Niki, Yoshitaka Tateishi, Manabu Ato, Sohkichi Matsumoto

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. The role of antibodies in tuberculosis diagnosis, prophylaxis and therapy: a review from the ESGMYC study group.European respiratory review : an official journal of the European Respiratory Society · 2022
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Desak Nyoman Surya Suameitria DewiUniversitas Airlangga, Faculty of Medicine, Doctoral Program of Medical Science, Surabaya, Indonesia. Electronic address: triacielo@gmail.com.
Ni Made MertaniasihUniversitas Airlangga, Faculty of Medicine, Department of Medical Microbiology, Surabaya, Indonesia; Universitas Airlangga, Institute of Tropical Disease, Laboratory of Tuberculosis, Surabaya, Indonesia. Electronic address: nmademertaniasih@gmail.com.
SoedarsonoUniversitas Airlangga, Faculty of Medicine, Department of Pulmonology and Respiratory Medicine, Surabaya, Indonesia. Electronic address: ssoedarsono@gmail.com.
Yuriko OzekiNiigata University, School of Medicine, Department of Bacteriology, Niigata, Japan. Electronic address: yuriozeki@med.niigata-u.ac.jp.
Wayan Tunas ArtamaUniversitas Gadjah Mada, Faculty of Veterinary Medicine, Department of Biochemistry, Yogyakarta, Indonesia; Universitas Gadjah Mada, One Health/Eco-health Resource Center, Yogyakarta, Indonesia. Electronic address: artama@ugm.ac.id.
FihiruddinUniversitas Gadjah Mada, Doctoral Program, Research Center of Biotechnology, Yogyakarta, Indonesia; Politeknik Kesehatan Mataram, Department of Medical Laboratory Technology, Mataram, Indonesia. Electronic address: fihir.analis@yahoo.co.id.
Mamiko NikiOsaka City University Graduate School of Medicine, Department of Bacteriology, Osaka, Japan. Electronic address: myoshi@med.osaka-cu.ac.jp.
Yoshitaka TateishiNiigata University, School of Medicine, Department of Bacteriology, Niigata, Japan. Electronic address: y-tateishi@med.niigata-u.ac.jp.
Manabu AtoNational Institute of Infectious Diseases, Department of Mycobacteriology, Leprosy Research Center, Tokyo, Japan. Electronic address: ato@niid.go.jp.
Sohkichi MatsumotoNiigata University, School of Medicine, Department of Bacteriology, Niigata, Japan; Universitas Airlangga, Faculty of Medicine, Department of Medical Microbiology, Surabaya, Indonesia. Electronic address: sohkichi@med.niigata-u.ac.jp.
Airlangga University · IDUniversitas Gadjah Mada · IDNational Institute of Infectious Diseases · JPNiigata University · JPNiigata University of Pharmacy and Medical and Life Sciences · JPOsaka City University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate and rapid diagnostic tools are important aspects of managing tuberculosis (TB) cases appropriately. However, the sensitivity and specificity of diagnostic kits based on immune response such as the tuberculin skin test (TST) and interferon gamma release assay (IGRA) are still debated. Thus, the exploration and assessment of specific biomarker-targeted antibodies are needed for the development of an accurate and rapid diagnostic tool. The present study was conducted in patients with a respiratory problem suspected to be TB at Dr. Soetomo Hospital, Surabaya, Indonesia. Among 102 patients tested by GeneXpert and AFB, 59 serum samples were from cases retrospectively determined to have active TB. A total of 102 serum of healthy controls (HC) was also collected. The PPD antigen and the recombinant CFP-10 and ESAT-6 proteins were prepared. Antibody responses against these proteins were evaluated by ELISA. All samples were also screened for the possibility of Mycobacterium avium-intracellulare complex (MAC) infection using Capilla MaC kit. The results showed that TB patients had a significantly higher concentration of IgG antibody in response to PPD than the HC. In addition, the receiver operating characteristic (ROC) curve analysis showed that PPD was acceptable for diagnostic purposes with an AUC value of 0.835 (95% CI 0.770-0.900, p < 0.0001). However, ESAT-6 and CFP-10 had low AUCs, and 32 samples from both groups showed a low concentration of IgA antibody against all antigens. The MAC detection results also showed that the concentration of IgA in the HC group was the highest. The current results indicate that PPD is a better antigen for antibody-based detection of TB than ESAT-6 and CFP-10. Based on the MAC detection assay, 53 people in the HC group were probably infected with rapidly growing nontuberculous mycobacteria (NTM), although antibody response to PPD was low.

Indexed as

AdolescentAdultAgedAntibody FormationAntigens, BacterialBacterial ProteinsCase-Control StudiesChildEnzyme-Linked Immunosorbent AssayFemaleHumansIndonesiaMaleMiddle AgedMycobacterium tuberculosisReference ValuesAntigens, BacterialBacterial ProteinsCFP-10 protein, Mycobacterium tuberculosisESAT-6 protein, Mycobacterium tuberculosisTuberculinAntibody response’ profileNTMPPDPulmonary tuberculosis

Identifiers

PMID31421107
PMCPMC9428029
OpenAlexW2968422496

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.