ArticlePloS one2019
Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Mito-nuclear incompatibility disrupts acyl-CoA oxidase 1/acyl-CoA oxidase 3-mediated mitochondria-peroxisome metabolic coordination in black soldier flies.Molecular biology and evolution · 2026Article
- Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in black soldier fly Hermetia illucens under different conditions.Insect science · 2026Article
- Reference gene selection for RT-qPCR normalization in the drone testis of honey bee (Apis cerana) during meiosis stages.PloS one · 2026Article
- Review
- The Black Soldier FlyCurrent research in microbial sciences · 2025Article
- Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells.Bone reports · 2024Article
- Stability and suitability of housekeeping genes in phlebotomine sand flies.Scientific reports · 2024Article
- Evaluation and Validation of Reference Genes for Gene Expression Analysis Using qRT-PCR in the Sugarcane Stem BorerInsects · 2024Article
- Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines.Scientific reports · 2023Article
- Enhanced protein degradation by black soldier fly larvae (Frontiers in microbiology · 2022Article
- Evaluation of Reference Genes inGenes · 2021Article
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- Reference gene screening for analyzing gene expression in the heart, liver, spleen, lung and kidney of forest musk deer.The Journal of veterinary medical science · 2021Article
- Detection of Pancreatic Ductal Adenocarcinoma by A qPCR-based Normalizer-free Circulating Extracellular Vesicles RNA Signature.Journal of Cancer · 2021Article
- Article
- Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae).Molecular biology reports · 2020Article
- Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).Scientific reports · 2020Article
- Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita.PloS one · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hermetia illucens is an important resource insect for the conversion of organic waste. Quantitative PCR (qPCR) is the primary tool of gene expression analysis and a core technology of molecular biology research. Reference genes are essential for qPCR analysis; however, a stability analysis of H. illucens reference genes has not yet been carried out. To find suitable reference genes for normalizing gene expression data, the stability of eight housekeeping genes (including ATP6V1A, RPL8, EF1, Tubulin, TBP, GAPDH, Actin and RP49) was investigated under both biotic (developmental stages, tissues and sex) and abiotic (heavy metals, food, antibiotics) conditions. Gene expression data were analysed by geNorm, NormFinder, BestKeeper, and ΔCt programs. A set of specific reference genes was recommended for each experimental condition using the results of RefFinder synthesis analysis. This study offers a solid foundation for further studies of the molecular biology of H. illucens.
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