ArticleMicrobiologyOpen2019
Rapid point-of-care diagnostics for the detection of Chlamydia pecorum in koalas (Phascolarctos cinereus) using loop-mediated isothermal amplification without nucleic acid purification.
Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Current status and future directions ofFrontiers in veterinary science · 2026Pooled it
- Koala Conservation: The Utility of Art and Genome Banking.Advances in experimental medicine and biology · 2026Review
- The relationships of viral and protozoal co-infections to Chlamydia pecorum infection and chlamydiosis outcomes in northern koalas (Phascolarctos cinereus).PLoS pathogens · 2025Article
- Evidence of Folliculogenesis and the Potential of Oocyte Recovery from Koalas with Different Levels of Reproductive Pathology.Biology · 2025Article
- Temporal dynamics of koala retrovirus plasma RNA load in relation to faecal glucocorticoid metabolites and Chlamydia infection.The Journal of general virology · 2025Article
- Dominance of Vaccine-SpecificEcology and evolution · 2025Article
- The Utility of the Koala Scat: A Scoping Review.Biology · 2024Article
- The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation.Biology of reproduction · 2023Article
- The Effect of Disease and Injury on Faecal Cortisol Metabolites, as an Indicator of Stress in Wild Hospitalised Koalas, Endangered Australian Marsupials.Veterinary sciences · 2023Article
- Development of diagnostic and point of care assays for a gammaherpesvirus infecting koalas.PloS one · 2023Article
- Article
- Development of multiplex HRM-based loop-mediated isothermal amplification method for specific and sensitive detection of Treponema pallidum.Archives of microbiology · 2022Article
- Koala retrovirus load and non-A subtypes are associated with secondary disease among wild northern koalas.PLoS pathogens · 2022Article
- Pharmacokinetic Profile of Doxycycline in Koala Plasma after Weekly Subcutaneous Injections for the Treatment of Chlamydiosis.Animals : an open access journal from MDPI · 2022Article
- Point-of-care testing in companion and food animal disease diagnostics.Frontiers in veterinary science · 2022Review
- Real-Time Fluorometric Isothermal LAMP Assay for Detection ofPathogens (Basel, Switzerland) · 2021Article
- Multiplex detection of blood-borne pathogens on a self-driven microfluidic chip using loop-mediated isothermal amplification.Analytical and bioanalytical chemistry · 2021Article
- Helping koalas battle disease - Recent advances in Chlamydia and koala retrovirus (KoRV) disease understanding and treatment in koalas.FEMS microbiology reviews · 2020Review
- Rapid point-of-care diagnostics for the detection of Chlamydia pecorum in koalas (Phascolarctos cinereus) using loop-mediated isothermal amplification without nucleic acid purification.MicrobiologyOpen · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious disease, predominately chlamydiosis, contributes significantly to the decline in health of wild koala (Phascolarctos cinereus) populations in some regions of Australia. In this study, we describe the development and evaluation of a simple, sensitive, and specific loop-mediated isothermal amplification (LAMP) assay for the detection of Chlamydia pecorum in koalas as a point-of-care diagnostic tool that can be used in any wildlife hospital and in the field on specialized instrumentation. A set of primers targeting a 188-bp region of the C. pecorum genome was designed. 100% specificity of the LAMP assay was revealed by demonstrating no cross-reactivity with 33 nontarget pathogens, and complete correlation with qPCR results for 43 clinical swabs collected opportunistically from wildlife hospitals. In sensitivity evaluations, the technique successfully detected serial dilutions of extracted C. pecorum DNA with a detection limit of 44 IFU/ml.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.