ArticleJournal of assisted reproduction and genetics2019
A treatment approach for couples with disrupted sperm DNA integrity and recurrent ART failure.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 3 syntheses or guidelines pooled it, 88 citations in OpenAlex.
- Pooled it
- Advanced Sperm Selection Strategies as a Treatment for Infertile Couples: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- What is the impact of granulocyte colony-stimulating factor (G-CSF) in subcutaneous injection or intrauterine infusion and during both the fresh and frozen embryo transfer cycles on recurrent implantation failure: a systematic review and meta-analysis?Reproductive biology and endocrinology : RB&E · 2021Pooled it
- Single-center thorough evaluation and targeted treatment of globozoospermic men.Journal of assisted reproduction and genetics · 2021Trial
- The impact of fresh versus cryopreserved testicular sperm on in-vitro fertilization outcomes in couples with elevated sperm DNA fragmentation: a retrospective cohort study.Reproductive biology and endocrinology : RB&E · 2026Article
- Evaluating Sperm DNA Damage: When and Why It Does Not Add to the Evaluation of Male Factor Infertility.Advances in experimental medicine and biology · 2026Review
- Sperm Selection Procedures in Cases of High Sperm DNA Fragmentation Index.Advances in experimental medicine and biology · 2026Review
- Review
- Comparison of Swim-Up and Microfluidic Sperm Sorting Methods in Selection of Sperm for Intracytoplasmic Sperm Injection.International journal of molecular sciences · 2025Article
- Microfluidic Sorting Can Be Applied for Assisted Reproduction Sperm Selection in Different Cases of Semen Abnormalities.Life (Basel, Switzerland) · 2025Article
- Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage.Life (Basel, Switzerland) · 2025Review
- The intricate "ART" of ICSI.Journal of assisted reproduction and genetics · 2025Review
- Sperm DNA Fragmentation and Fertility.Advances in experimental medicine and biology · 2025Review
- Embryology outcomes of a device-based sperm separation technique compared to density gradient centrifugation using thawed spermatozoa-a sibling donor oocyte study.Journal of assisted reproduction and genetics · 2025Article
- Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes.Animals : an open access journal from MDPI · 2024Article
- Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index.Journal of assisted reproduction and genetics · 2024Observational
- Automated Single-Sperm Selection Software (SiD) during ICSI: A Prospective Sibling Oocyte Evaluation.Medical sciences (Basel, Switzerland) · 2024Article
- Advanced Paternal Age: A New Indicator for the Use of Microfluidic Devices for Sperm DNA Fragmentation Selection.Journal of clinical medicine · 2024Article
- Can a sperm selection technique improve embryo ploidy?Andrology · 2023Article
- Association of embryo aneuploidy and sperm DNA damage in unexplained recurrent implantation failure patients under NGS-based PGT-A cycles.Archives of gynecology and obstetrics · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo test a novel method to select spermatozoa with high chromatin integrity.
designSpecimens with high sperm chromatin fragmentation (SCF) were selected by density gradient selection (DGS) and microfluidic sperm sorting (MSS).
settingAcademic medical center. PATIENT(S): Ejaculates from consenting men were processed by DGS/MSS. Couples underwent ICSI cycles with spermatozoa processed by DGS/MSS. Clinical outcomes were evaluated after embryo transfer. INTERVENTION(S): SCF was measured by TUNEL. ICSI with spermatozoa selected by DGS and MSS was performed. MAIN OUTCOME MEASURE(S): Fertilization, embryo implantation, and pregnancy outcomes were compared between DGS and MSS. RESULT(S): A total of 23 men had an average SCF of 20.7 ± 10%. After DGS and MSS, the SCF was 12.5 ± 5% and 1.8 ± 1%, respectively. In couples who underwent ICSI, the average SCF was 28.8 ± 9%, which fell to 21.0 ± 9% after DGS and 1.3 ± 0.7% after MSS. Four couples underwent 11 ICSI cycles with DGS and achieved one (25%) pregnancy that resulted in pregnancy loss. In four subsequent ICSI cycles with MSS, an ongoing clinical pregnancy rate of 50% was achieved. Five additional couples underwent 12 cycles of ICSI with DGS. After preimplantation genetic testing for aneuploidy, 30.3% of the embryos were euploid. One pregnancy was achieved, resulting in pregnancy loss. With MSS, 31.5% of the embryos were euploid and 4 couples obtained a pregnancy. Finally, sixteen couples underwent 20 ICSI cycles solely with MSS at our center. Of these couples, 8 had failed 13 ICSI cycles with DGS elsewhere. These couples achieved an overall implantation of 34.5% (10/29) and a pregnancy rate of 58.8% (10/17). CONCLUSION(S): Microfluidic selection yielded spermatozoa with optimal genomic integrity and improved chances of obtaining a euploid conceptus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.